Ma Jie, Gao Hai-Mei, Hua Xin, Lu Ze-Yuan, Gao Hai-Cheng
Department of Molecular Medicine, Jilin University, Changchun, China E-mail :
Asian Pac J Cancer Prev. 2014;15(9):4045-8. doi: 10.7314/apjcp.2014.15.9.4045.
Effects of transforming growth factor-beta (TGF-β) were investigated in human colorectal cancer, and the influence of cantharidinate in inhibiting TGF-β1 expression was explored. Relationships among TGF-β1 and sex, age, tumor size, tumor location, tumor stage were also analyzed. H and E and immunohistochemistry staining were employed to assess colorectal cancer and TGF-β1 expression, respectively. Then, HCT-116 CRC cells were randomly divided into four groups, controls, no serum-treated, chemotherapy and cantharidinate-treated. Immunohistochemistry and real-time PCR were employed to assess the expression of TGF-β1 in CRC cells. Our data showed that the expression of TGF-β1 might be associated with tumor size and tumor location (P<0.05). The expression of TGF-β1 in CRC groups was higher than in adjacent groups (P<0.05). In addition, the expression of TGF-β1 in cantharidinate-treated group was much lower than in CRC group (P<0.05). Taken together, these results suggest that TGF-β1 plays an important role in CRC development. Cantharidinate might inhibit the expression of TGF-β1 and control the development of colorectal cancer.
研究了转化生长因子-β（TGF-β）在人类结直肠癌中的作用，并探讨了斑蝥素对抑制TGF-β1表达的影响。还分析了TGF-β1与性别、年龄、肿瘤大小、肿瘤位置、肿瘤分期之间的关系。分别采用苏木精-伊红染色（H&E）和免疫组织化学染色来评估结直肠癌和TGF-β1的表达。然后，将HCT-116结直肠癌细胞随机分为四组，即对照组、无血清处理组、化疗组和斑蝥素处理组。采用免疫组织化学和实时定量聚合酶链反应（PCR）来评估结直肠癌细胞中TGF-β1的表达。我们的数据表明，TGF-β1的表达可能与肿瘤大小和肿瘤位置有关（P<0.05）。结直肠癌组中TGF-β1的表达高于相邻组（P<0.05）。此外，斑蝥素处理组中TGF-β1的表达远低于结直肠癌组（P<0.05）。综上所述，这些结果表明TGF-β1在结直肠癌的发生发展中起重要作用。斑蝥素可能抑制TGF-β1的表达并控制结直肠癌的发展。
