Liu Bin, Gao Hai-Cheng, Xu Jing-Wei, Cao Hong, Fang Xue-Dong, Gao Hai-Mei, Qiao Shi-Xing
Department of General Surgery, Second Hospital of Jilin University, Changchun, China.
Asian Pac J Cancer Prev. 2012;13(8):3705-8. doi: 10.7314/apjcp.2012.13.8.3705.
Effects of Cantharidinate on apoptosis of human colorectal cancer UTC-116 cells were investigated by means of 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, H and E staining, flow cytometry, and Raman Spectra analysis. The results showed Cantharidinate to exert inhibitory action on proliferation of human colorectal cancer UTC-116 cells, inducing apoptosis, arresting cells in G1 phase, with decline of S and G2 phases. In addition, the results of Raman spectrum showed significant changes in the UTC-116 cells chemical structure with stretching after the application of Cantharidinate. Taken together, these results suggest that the treatment of human colorectal cancer with Cantharidinate may be associated with multiple molecular mechanisms for apoptosis. Furthermore, similar to fluorouracil, Cantharidinate should be considered as novel assistant drug for controlling the growth of human colorectal cancer UTC-116 cells.
通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法、苏木精和伊红（H&E）染色、流式细胞术以及拉曼光谱分析，研究了斑蝥素对人结直肠癌UTC-116细胞凋亡的影响。结果表明，斑蝥素对人结直肠癌UTC-116细胞的增殖具有抑制作用，可诱导细胞凋亡，使细胞停滞于G1期，S期和G2期细胞减少。此外，拉曼光谱结果显示，应用斑蝥素后，UTC-116细胞的化学结构在拉伸方面发生了显著变化。综上所述，这些结果表明，斑蝥素治疗人结直肠癌可能与多种凋亡分子机制有关。此外，与氟尿嘧啶类似，斑蝥素应被视为控制人结直肠癌UTC-116细胞生长的新型辅助药物。
