Haq Ihsan ul, Vreysen Marc J B, Cacéres Carlos, Shelly Todd E, Hendrichs Jorge
Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Seibersdorf, Austria.
National Agricultural Research Centre, Park Road Islamabad, Pakistan.
Insect Sci. 2015 Oct;22(5):661-9. doi: 10.1111/1744-7917.12148. Epub 2014 Nov 11.
Methyl-eugenol (ME) (1,2-dimethoxy-4-(2-propenyl)benzene), a natural phytochemical, did enhance male Bactrocera carambolae Drew & Hancock (Diptera: Tephritidae) mating competitiveness 3 d after ingestion. Enhanced male mating competitiveness can significantly increase the effectiveness of the sterile insect technique (SIT). ME application to mass reared sterile flies by feeding is infeasible. ME application by aromatherapy however, would be a very practical way of ME application in fly emergence and release facilities. This approach was shown to enhance mating competitiveness of B. carambolae 3 d posttreatment (DPT). Despite this added benefit, every additional day of delaying release will reduce sterile fly quality and will add cost to SIT application. The present study was planned to assess the effects of ME-aromatherapy on male B. carambolae mating competitiveness 1DPT and 2DPT. ME aromatherapy 1DPT or 2DPT did enhance mating competitiveness of B. carambolae males whereas ME feeding 1DPT and 2DPT did not. Male mating competitiveness was enhanced by the ME aromatherapy irrespective if they received 1DPT, 2DPT or 3DPT. ME aromatherapy, being a viable approach for its application, did enhance mating competitiveness of male B. carambolae 1 d posttreatment as ME feeding did 3 d after ingestion.
甲基丁香酚（ME）（1,2 - 二甲氧基 - 4 - （2 - 丙烯基）苯）是一种天然植物化学物质，在雄虫摄入后3天确实增强了杨桃实蝇Drew & Hancock（双翅目：实蝇科）的交配竞争力。增强的雄虫交配竞争力可显著提高昆虫不育技术（SIT）的有效性。通过喂食将ME应用于大规模饲养的不育果蝇是不可行的。然而，通过芳香疗法应用ME，将是在果蝇羽化和释放设施中应用ME的一种非常实用的方法。这种方法被证明在处理后3天（DPT）可增强杨桃实蝇的交配竞争力。尽管有这一额外益处，但每延迟一天释放都会降低不育果蝇的质量，并增加昆虫不育技术应用的成本。本研究旨在评估ME芳香疗法在处理后1天（DPT）和2天（DPT）对杨桃实蝇雄虫交配竞争力的影响。处理后1天或2天进行ME芳香疗法确实增强了杨桃实蝇雄虫的交配竞争力，而处理后1天和2天进行ME喂食则没有。无论雄虫接受处理后1天、2天还是3天的ME芳香疗法，其交配竞争力均得到增强。ME芳香疗法作为一种可行的应用方法，在处理后1天确实增强了杨桃实蝇雄虫的交配竞争力，就像雄虫摄入ME后3天通过喂食增强交配竞争力一样。
