Wang Xiaojia, Liu Wenbin, Zhu Dekang, Yang LinFeng, Liu MaFeng, Yin Sanjun, Wang MingShu, Jia RenYong, Chen Shun, Sun KunFeng, Cheng Anchun, Chen Xiaoyue
Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, 46# Xinkang Road, Ya'an, Sichuan 625014, P,R, China.
BMC Genomics. 2014 Jun 17;15(1):479. doi: 10.1186/1471-2164-15-479.
Riemerella anatipestifer is one of the most important pathogens of ducks. However, the molecular mechanisms of R. anatipestifer infection are poorly understood. In particular, the lack of genomic information from a variety of R. anatipestifer strains has proved severely limiting.
In this study, we present the complete genomes of two R. anatipestifer strains, RA-CH-1 (2,309,519 bp, Genbank accession CP003787) and RA-CH-2 (2,166,321 bp, Genbank accession CP004020). Both strains are from isolates taken from two different sick ducks in the SiChuang province of China. A comparative genomics approach was used to identify similarities and key differences between RA-CH-1 and RA-CH-2 and the previously sequenced strain RA-GD, a clinical isolate from GuangDong, China, and ATCC11845.
The genomes of RA-CH-2 and RA-GD were extremely similar, while RA-CH-1 was significantly different than ATCC11845. RA-CH-1 is 140,000 bp larger than the three other strains and has 16 unique gene families. Evolutionary analysis shows that RA-CH-1 and RA-CH-2 are closed and in a branch with ATCC11845, while RA-GD is located in another branch. Additionally, the detection of several iron/heme-transport related proteins and motility mechanisms will be useful in elucidating factors important in pathogenicity. This information will allow a better understanding of the phenotype of different R. anatipestifer strains and molecular mechanisms of infection.
鸭疫里默氏菌是鸭最重要的病原体之一。然而，鸭疫里默氏菌感染的分子机制尚不清楚。特别是，缺乏来自多种鸭疫里默氏菌菌株的基因组信息已被证明是严重的限制因素。
在本研究中，我们展示了两株鸭疫里默氏菌菌株RA-CH-1（2,309,519 bp，Genbank登录号CP003787）和RA-CH-2（2,166,321 bp，Genbank登录号CP004020）的完整基因组。这两株菌株均来自中国四川省两只不同病鸭的分离株。采用比较基因组学方法来鉴定RA-CH-1和RA-CH-2与先前测序的菌株RA-GD（一株来自中国广东的临床分离株）以及ATCC11845之间的异同。
RA-CH-2和RA-GD的基因组极其相似，而RA-CH-1与ATCC11845有显著差异。RA-CH-1比其他三株菌株大140,000 bp，并且有16个独特的基因家族。进化分析表明，RA-CH-1和RA-CH-2关系密切，与ATCC11845处于一个分支，而RA-GD位于另一个分支。此外，对几种铁/血红素转运相关蛋白和运动机制的检测将有助于阐明致病性中的重要因素。这些信息将有助于更好地理解不同鸭疫里默氏菌菌株的表型和感染的分子机制。
