Witt Benjamin L, Albertson Daniel J, Coppin Margaret G, Horrocks Christian F, Post Melissa, Gulbahce H Evin
Department of Pathology, University of Utah School of Medicine, 1950 Circle of Hope, Rm N3100, Salt Lake City, UT, 84112, USA.
Head Neck Pathol. 2015 Mar;9(1):60-4. doi: 10.1007/s12105-014-0549-1. Epub 2014 Jun 17.
The human papillomavirus (HPV) status of head and neck squamous cell carcinomas (SCCs) is a frequent request for Anatomic Pathology labs. However, prognostic value of HPV status is limited to identification of high risk HPV in oropharyngeal SCCs. The purpose of this study is to investigate the ordering practices of in situ hybridization (ISH) for HPV in head and neck tissues at our national reference laboratory. All testing orders for low risk, high risk, and combined low and high risk HPV-ISH tests requested at ARUP Laboratories between January 2010 and November 2013 had their results reviewed and were grouped by anatomic location of the tested tissue. The H&E and HPV-ISH slides from a sample of the most recent 123 tests were reviewed by two pathologists. A total of 1,128 HPV-ISH tests were ordered during the study period. Testing for combined low and high risk HPV was the most commonly ordered test. The positivity rate for high risk HPV was highest in oropharyngeal tissues. 49 of 123 reviewed cases had testing requested on non-malignant tissue, 11 of which were non-neoplastic. Unnecessary HPV-ISH ordering is prevalent in head and neck tissues. Dual testing for low and high risk HPV, frequent testing outside of the oropharynx, and testing non-neoplastic tissues appear to be common practices.
对头颈部鳞状细胞癌（SCC）进行人乳头瘤病毒（HPV）检测是解剖病理学实验室经常接到的请求。然而，HPV状态的预后价值仅限于口咽SCC中高危HPV的识别。本研究的目的是调查我们国家参考实验室对头颈部组织进行HPV原位杂交（ISH）检测的送检情况。对2010年1月至2013年11月期间在ARUP实验室请求的所有低危、高危以及低危和高危HPV-ISH联合检测的订单结果进行审查，并按检测组织的解剖位置进行分组。两位病理学家对最近123次检测样本的苏木精-伊红（H&E）和HPV-ISH切片进行了审查。在研究期间共下达了1128次HPV-ISH检测订单。低危和高危HPV联合检测是最常下达的检测项目。高危HPV的阳性率在口咽组织中最高。在123例审查病例中，有49例对非恶性组织进行了检测请求，其中11例为非肿瘤性组织。在头颈部组织中，不必要的HPV-ISH送检很普遍。对低危和高危HPV进行双重检测、在口咽以外频繁检测以及检测非肿瘤组织似乎是常见做法。
