Yıldırım Ali Osman, Eryılmaz Mehmet, Kaldırım Umit, Eyi Yusuf Emrah, Tuncer Salim Kemal, Eroğlu Murat, Durusu Murat, Topal Turgut, Kurt Bülent, Dilmen Serkan, Bilgiç Serkan, Serdar Muhittin
Department of Emergency Medicine, GATA, Haydarpasa Military Hosital, İstanbul, Turkey.
Department of Emergency Medicine, GATA School of Medicine, Ankara, Turkey.
Ulus Travma Acil Cerrahi Derg. 2014 May;20(3):167-75. doi: 10.5505/tjtes.2014.09465.
Soft tissue trauma is a type of acute traumatic ischemia. We investigated in this study whether the edema, inflammation and ischemia caused by the trauma could be affected positively by hyperbaric oxygen (HBO) and ozone therapy.
Soft tissue trauma was generated in a total of 63 adult male Sprague-Dawley rats. Subsequently, rats were divided into three groups. The first group was treated with ozone, the second group with HBO, and the third group served as controls. Tissue and blood samples were taken at the end of the procedures. Tissue lipid peroxidation (LPO), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), inducible nitric oxide synthase (iNOS), heme oxygenase (HO)-1, and hypoxia-inducible factor (HIF)-1 levels were detected. Hematoxylin-eosin staining was used to determine the inflammation and edema histopathologically.
We also detected HIF-1 activity, which decreases when the oxygen concentration increases, HO-1 activity, which has anti-inflammatory effects, and iNOS activity, which releases in any type of acute case. We determined a statistically significant reduction in iNOS and LPO levels in both the HBO and Ozone groups. A significant decrease in inflammation was detected in both the Ozone and HBO groups compared with the Control group, and a significant decrease in edema was detected in all three groups.
We think that HBO and Ozone therapy have beneficial effects on biochemical and histopathological findings. Related clinical trials will be helpful in clarifying the effects.
软组织创伤是一种急性创伤性缺血。在本研究中，我们调查了创伤引起的水肿、炎症和缺血是否会受到高压氧（HBO）和臭氧疗法的积极影响。
总共63只成年雄性Sprague-Dawley大鼠制造软组织创伤。随后，将大鼠分为三组。第一组用臭氧治疗，第二组用HBO治疗，第三组作为对照组。在实验结束时采集组织和血液样本。检测组织脂质过氧化（LPO）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、诱导型一氧化氮合酶（iNOS）、血红素加氧酶（HO）-1和缺氧诱导因子（HIF）-1水平。苏木精-伊红染色用于病理组织学确定炎症和水肿情况。
我们还检测到了HIF-1活性（氧浓度增加时会降低）、具有抗炎作用的HO-1活性以及在任何急性病例中都会释放的iNOS活性。我们确定HBO组和臭氧组的iNOS和LPO水平均有统计学意义的降低。与对照组相比，臭氧组和HBO组的炎症均有显著降低，且三组的水肿均有显著降低。
我们认为HBO和臭氧疗法对生化和组织病理学结果有有益影响。相关临床试验将有助于阐明其效果。
