Koçyiğit Aytuğ, Gülmen Şenol, Kurtoğlu Tunay, Kumbul Doğuç Duygu, Meteoğlu İbrahim, Okutan Hüseyin, Alkaya Solmaz Filiz
Department of Cardiovascular Surgery, Private Denizli Tekten Hospital, Denizli, Turkey.
Department of Cardiovascular Surgery, Medicine Faculty of Süleyman Demirel University, Isparta, Turkey.
Turk Gogus Kalp Damar Cerrahisi Derg. 2018 Apr 30;26(2):207-213. doi: 10.5606/tgkdc.dergisi.2018.15484. eCollection 2018 Apr.
This study aims to investigate the effect of ozone on myocardial ischemia-reperfusion injury occurring after occlusion - reperfusion of infrarenal abdominal aorta in rats.
Thirty-two Wistar albino rats (weighing 200-250 g) were randomized into four equal groups. The control (sham) group underwent laparotomy and dissection of the infrarenal abdominal aorta without occlusion. Intraperitoneal ozone was applied for 10 days 1 mg/kg/day in the control+ozone group. Afterwards, control+ozone group underwent laparotomy and dissection of the infrarenal abdominal aorta without occlusion. Aortic ischemia-reperfusion and aortic ischemia-reperfusion+ozone groups underwent dissection of the infrarenal abdominal aorta, followed by achieving ischemia and reperfusion by cross-clamping the infrarenal abdominal aorta for 60 minutes and removing the cross-clamp for 60 minutes, respectively. The tissue levels of malondialdehyde and activity levels of superoxide dismutase, catalase, and myeloperoxidase were measured in the myocardial specimens. The tumor necrosis factor, interleukin-6 and troponin-I levels were measured in the plasma. A histopathological examination of the myocardial specimens was undertaken.
Biochemical analysis showed that aortic ischemia-reperfusion significantly increased (p<0.05 vs. control) while ozone significantly decreased (p<0.05 vs. aortic ischemia-reperfusion) the myocardial tissue levels of superoxide dismutase and catalase and level of plasma troponin-I. Histologically, in the aortic ischemia-reperfusion group, myocardial disorganization, myofiber swelling and myofiber eosinophilia in the myocardial tissue samples were significantly increased compared to the control group (p<0.05 vs. control). However, histopathological changes in the aortic ischemia-reperfusion+ozone group decreased compared to the aortic ischemia-reperfusion group.
The results of this experimental study indicate that ozone attenuates myocardial injury and oxidative stress that develop after infrarenal aortic ischemia-reperfusion through three markers; (i) decreased tissue superoxide dismutase and catalase levels, (ii) d ecreased p lasma t roponin-I l evels, a nd (iii) reduced histopathological changes, albeit not statistically significant.
本研究旨在探讨臭氧对大鼠肾下腹主动脉阻断 - 再灌注后发生的心肌缺血 - 再灌注损伤的影响。
32只Wistar白化大鼠（体重200 - 250克）被随机分为四组，每组数量相等。对照组（假手术组）进行剖腹术并解剖肾下腹主动脉但不阻断。对照组 + 臭氧组腹腔注射臭氧10天，剂量为1毫克/千克/天。之后，对照组 + 臭氧组进行剖腹术并解剖肾下腹主动脉但不阻断。主动脉缺血 - 再灌注组和主动脉缺血 - 再灌注 + 臭氧组进行肾下腹主动脉解剖，然后分别通过夹闭肾下腹主动脉60分钟造成缺血，松开夹闭60分钟实现再灌注。检测心肌标本中丙二醛的组织水平以及超氧化物歧化酶、过氧化氢酶和髓过氧化物酶的活性水平。检测血浆中肿瘤坏死因子、白细胞介素 - 6和肌钙蛋白 - I的水平。对心肌标本进行组织病理学检查。
生化分析表明，主动脉缺血 - 再灌注显著增加（与对照组相比，p < 0.05），而臭氧显著降低（与主动脉缺血 - 再灌注组相比，p < 0.05）心肌组织中超氧化物歧化酶和过氧化氢酶的水平以及血浆肌钙蛋白 - I的水平。组织学上，与对照组相比，主动脉缺血 - 再灌注组心肌组织样本中的心肌紊乱、肌纤维肿胀和肌纤维嗜酸性显著增加（与对照组相比，p < 0.05）。然而，与主动脉缺血 - 再灌注组相比，主动脉缺血 - 再灌注 + 臭氧组的组织病理学变化有所减少。
本实验研究结果表明，臭氧通过三个指标减轻肾下腹主动脉缺血 - 再灌注后发生的心肌损伤和氧化应激：（i）组织中超氧化物歧化酶和过氧化氢酶水平降低；（ii）血浆肌钙蛋白 - I水平降低；（iii）组织病理学变化减少，尽管差异无统计学意义。
