Park Jun Hee, Cho Yea Seul, Kang Sungmuk, Lee Eun Jeong, Lee Gwan-Ho, Hah Sang Soo
Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea.
Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea.
Anal Biochem. 2014 Oct 1;462:10-2. doi: 10.1016/j.ab.2014.05.015. Epub 2014 Jun 14.
A colorimetric sandwich-type assay based on enzyme-linked aptamer assay has been developed for the fast and sensitive detection of as low as 25 fM of thrombin with high linearity. Aptamer-immobilized glass was used to capture the target analyte, whereas a second aptamer, functionalized with horseradish peroxidase (HRP), was employed for the conventional 3,5,3',5'-tetramethylbenzidine (TMB)-based colorimetric detection. Without the troublesome antibody requirement of the conventional enzyme-linked immunosorbent assay (ELISA), as low as 25 fM of thrombin could be rapidly and reproducibly detected. This assay has superior, or at least equal, recovery and accuracy to that of conventional antibody-based ELISA.
基于酶联适配体分析开发了一种比色夹心型分析方法，可快速、灵敏地检测低至25 fM的凝血酶，且具有高线性。固定有适配体的玻璃用于捕获目标分析物，而用辣根过氧化物酶（HRP）功能化的第二种适配体用于基于传统3,5,3',5'-四甲基联苯胺（TMB）的比色检测。该方法无需传统酶联免疫吸附测定（ELISA）中麻烦的抗体，可快速、可重复地检测低至25 fM的凝血酶。该分析方法的回收率和准确性优于或至少等同于传统基于抗体的ELISA。
