Omotoso G O, Ibitolu J O, Femi-Akinlosotu O M, Akinola O B, Enaibe B U
Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin.
Niger J Physiol Sci. 2013 Dec 20;28(2):147-51.
Tobacco smoking has been linked to many preventable diseases affecting various organs and systems of the body, including the brain. The current study was conducted to demonstrate the histological changes observable in the cerebral cortex of young Wistar rats exposed to nicotine during gestation. Vaginal smearing was conducted for the female Wistar rats to determine their oestrous cycle, after which they were exposed to male rats overnight, for mating. Pregnancy was confirmed and the pregnant rats were divided into 3 groups based on the 3 trimesters (A, B, C), with each group having a control and a treated subgroup. The Control Groups (A1, B1, C1) were given 0.1 ml of normal saline i. p., while the Treated Groups (A2, B2, C2) received 0.06 mg/kg/0.1 ml of nicotine intra-peritoneally. Treatment was for a period of 6 days only within each trimester for all subgroups. The pregnant animals were allowed to litter, and at postnatal day 35 they were sacrificed. The skull was dissected to expose and remove the brain; the temporal and parietal cortices were excised and fixed in 4% paraformaldehyde for histological tissue preparation, using cresyl fast violet staining techniques. Exposure of the developing brain to nicotine during gestation resulted in various degrees of abnormalities in the cytoarchitecture of the parietal and temporal cortices of young rats. The gestational period of nicotine exposure and specific cortical affectation are important factors to consider while investigating neurological abnormalities in offspring of tobacco smokers.
吸烟与许多可预防的疾病有关，这些疾病会影响身体的各个器官和系统，包括大脑。本研究旨在证明妊娠期间接触尼古丁的幼年Wistar大鼠大脑皮层中可观察到的组织学变化。对雌性Wistar大鼠进行阴道涂片以确定其发情周期，之后让它们与雄性大鼠过夜接触以进行交配。确认怀孕后，将怀孕大鼠根据三个孕期分为3组（A、B、C），每组有一个对照组和一个处理组。对照组（A1、B1、C1）腹腔注射0.1 ml生理盐水，而处理组（A2、B2、C2）腹腔注射0.06 mg/kg/0.1 ml尼古丁。所有亚组在每个孕期仅进行为期6天的处理。让怀孕动物产仔，在出生后第35天对它们实施安乐死。解剖颅骨以暴露并取出大脑；切除颞叶和顶叶皮层，并使用甲酚紫染色技术将其固定在4%多聚甲醛中以制备组织学标本。妊娠期间发育中的大脑接触尼古丁会导致幼鼠顶叶和颞叶皮层的细胞结构出现不同程度的异常。在调查吸烟者后代的神经异常时，接触尼古丁的妊娠期和特定皮层影响是需要考虑的重要因素。
