Otto W, Sinell H J
Berl Munch Tierarztl Wochenschr. 1989 Jan 1;102(1):14-8.
Addition of blood plasma to meat products is not permitted in the FRG unless these products are heat processed using an internal temperature of 80 degrees C (German regulation of meat and meat products: "Verordnung für Fleisch und Fleischerzeugnisse"). Such heat process may have an unfavourable effect on the detectability of blood plasma. Since blood plasma or dried plasma may originate from different animal species (porcine or bovine) two different anti dried blood plasma-sera (porcine and bovine) are required for immunochemical analysis. The varying quality of these sera has to be considered when interpreting the results. Seven M urea extract turned out to be suitable for detection of dried plasma additives and proved to be highly effective particularly when examining heated samples. Both the gel-diffusion and the electro immuno assay proved useful for the detection of dried blood plasma, provided the examined extracts had been adequately diluted. Immunochemical reactivity was hampered by the heat process which was given to the sample. Accordingly, the concentration of the plasma in a particular sample cannot be determined unless the time/temperature data of the process applied to the sample were given and model samples were tested for comparison.
在联邦德国，向肉制品中添加血浆是不被允许的，除非这些产品采用80摄氏度的内部温度进行热处理（德国肉类和肉制品法规：“关于肉类和肉类制品的条例”）。这样的热处理可能会对血浆的可检测性产生不利影响。由于血浆或干血浆可能来源于不同的动物物种（猪或牛），免疫化学分析需要两种不同的抗干血浆血清（猪和牛）。在解释结果时必须考虑这些血清质量的差异。结果表明，7M尿素提取物适用于检测干血浆添加剂，尤其在检测加热后的样品时证明非常有效。凝胶扩散法和电免疫分析法都被证明对检测干血浆有用，前提是所检测的提取物经过了充分稀释。样品经过的热处理会阻碍免疫化学反应。因此，除非给出应用于样品的处理时间/温度数据并测试模型样品进行比较，否则无法确定特定样品中血浆的浓度。
