Yang Lei, Naylor Gavin J P
a Department of Biology , College of Charleston , Charleston , SC , USA.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):943-4. doi: 10.3109/19401736.2014.926497. Epub 2014 Jun 18.
We determined the complete mitochondrial genome sequence (16,760 bp) of the peacock skate Pavoraja nitida using a long-PCR based next generation sequencing method. It has 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region in the typical vertebrate arrangement. Primers, protocols, and procedures used to obtain this mitogenome are provided. We anticipate that this approach will facilitate rapid collection of mitogenome sequences for studies on phylogenetic relationships, population genetics, and conservation of cartilaginous fishes.
我们使用基于长PCR的下一代测序方法，测定了孔雀鳐（Pavoraja nitida）的完整线粒体基因组序列（16,760 bp）。它具有典型脊椎动物排列方式的13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和1个控制区。文中提供了用于获取该线粒体基因组的引物、方案和步骤。我们预计这种方法将有助于快速收集线粒体基因组序列，以用于软骨鱼类的系统发育关系、群体遗传学和保护研究。
