Jeong Dageum, Lee Youn-Ho
Marine Ecosystem Research Division, Korea Institute of Ocean Science & Technology , Sangrok-gu, Ansan , Republic of Korea and.
Mitochondrial DNA. 2015 Feb;26(1):123-4. doi: 10.3109/19401736.2013.814113. Epub 2013 Jul 24.
The complete sequence of mitochondrial DNA of a longnose skate, Raja rhina was determined for the first time. It is 16,910 bp in length containing 2 rRNA, 22 tRNA and 13 protein coding genes with the same gene order and structure as those of other Rajidae species. The nucleotide of L-strand is composed of 30.1% A, 27.2% C, 28.5% T and 14.2% G, showing a slight A + T bias. The G is the least used base and markedly lower at the third codon position (5.4%). Twelve of the 13 protein coding genes use ATG as their start codon while the COX1 starts with GTG. As for stop codon, only ND4 shows incomplete stop codon TA. This mitogenome is the first report for a species of the genus Raja, and providing a valuable resource of genetic information for understanding the phylogenetic relationship and the evolution of the genus Raja as well as the family, Rajidae.
首次测定了长吻鳐（Raja rhina）线粒体DNA的完整序列。其长度为16910 bp，包含2个rRNA、22个tRNA和13个蛋白质编码基因，基因顺序和结构与其他鳐科物种相同。轻链的核苷酸由30.1%的A、27.2%的C、28.5%的T和14.2%的G组成，显示出轻微的A+T偏向。G是使用最少的碱基，在第三密码子位置明显较低（5.4%）。13个蛋白质编码基因中有12个使用ATG作为起始密码子，而COX1以GTG开头。至于终止密码子，只有ND4显示不完全终止密码子TA。这个线粒体基因组是鳐属物种的首次报道，为理解鳐属以及鳐科的系统发育关系和进化提供了宝贵的遗传信息资源。
