家鸡（鸡形目：雉科）的完整线粒体基因组
Complete mitochondrial genome of Gallus domesticus (Galliformes: Phasianidae).
作者信息
Wang Shanghong, Wang Binhua, Wang Fang, Wu Zhiqiang
机构信息
a Department of Biological Science , Nanchang University , Nanchang , China and.
b Department of Environmental Engineering , Guilin University of Technology , Guilin , China.
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):978-9. doi: 10.3109/19401736.2014.926514. Epub 2014 Jun 18.
In this paper, the complete mitochondrial genome (mitogenome) sequence of Gallus domesticus was determined by long PCR and primer walking methods. The complete mitochondrial genome is 16,783 bp in length and contains 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes as well as a displacement loop (D-loop). The overall base composition of the genome is A(30.25%), T(23.79%), C(32.44%), G(13.52%), respectively. The mitogenome of G. domesticus displayed novel gene order arrangement compared with published Gallus gallus var. domesticus to date. The mitogenome would contribute to resolving phylogenetic position and interrelationships of Gallus.
本文采用长PCR和引物步移法测定了家鸡的完整线粒体基因组（线粒体基因组）序列。完整的线粒体基因组长度为16783bp，包含13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因以及一个位移环（D环）。基因组的总体碱基组成为A（30.25%）、T（23.79%）、C（32.44%）、G（13.52%）。与迄今为止已发表的原鸡家鸡变种相比，家鸡的线粒体基因组显示出新颖的基因顺序排列。该线粒体基因组将有助于解决家鸡的系统发育位置和种间关系。
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