Kettlitz Siegfried W, Moosmann Carola, Valouch Sebastian, Lemmer Uli
Light Technology Institute and Institute of Microstructure Technology, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Cytometry A. 2014 Sep;85(9):746-55. doi: 10.1002/cyto.a.22499. Epub 2014 Jun 17.
Microfluidic flow cytometers are highly interesting candidates for biomedical point-of-care applications. However, the sensitivity, reliability, and throughput of these systems must be improved to provide the full functionality of established flow cytometric systems. One proposed method to improve fluorescence detection systems is to use spatial modulation techniques. We derive the noise-related statistics and calculate the coefficient of variation for a detection system with and without spatial modulation. We measure the noise properties of a nonmodulated microfluidic fluorescence particle detection system and analyze the possible performance gains using spatial modulation.
微流控流式细胞仪是生物医学即时检测应用中极具吸引力的候选设备。然而，这些系统的灵敏度、可靠性和通量必须加以提高，才能提供成熟流式细胞仪系统的全部功能。一种提高荧光检测系统性能的方法是采用空间调制技术。我们推导了与噪声相关的统计数据，并计算了有空间调制和无空间调制时检测系统的变异系数。我们测量了非调制微流控荧光颗粒检测系统的噪声特性，并分析了采用空间调制可能带来的性能提升。
