Center for Biomedical Engineering, University of New Mexico, Albuquerque, NM, USA.
Lab Chip. 2014 Mar 21;14(6):1044-59. doi: 10.1039/c3lc51152a.
A modern flow cytometer can analyze and sort particles on a one by one basis at rates of 50,000 particles per second. Flow cytometers can also measure as many as 17 channels of fluorescence, several angles of scattered light, and other non-optical parameters such as particle impedance. More specialized flow cytometers can provide even greater analysis power, such as single molecule detection, imaging, and full spectral collection, at reduced rates. These capabilities have made flow cytometers an invaluable tool for numerous applications including cellular immunophenotyping, CD4+ T-cell counting, multiplex microsphere analysis, high-throughput screening, and rare cell analysis and sorting. Many bio-analytical techniques have been influenced by the advent of microfluidics as a component in analytical tools and flow cytometry is no exception. Here we detail the functions and uses of a modern flow cytometer, review the recent and historical contributions of microfluidics and microfabricated devices to field of flow cytometry, examine current application areas, and suggest opportunities for the synergistic application of microfabrication approaches to modern flow cytometry.
现代流式细胞仪可以以每秒 50,000 个颗粒的速度逐个分析和分选颗粒。流式细胞仪还可以测量多达 17 个荧光通道、几个角度的散射光以及其他非光学参数，如颗粒阻抗。更专业的流式细胞仪可以提供更高的分析能力，例如单分子检测、成像和全光谱采集，但其速度会降低。这些功能使流式细胞仪成为许多应用的宝贵工具，包括细胞免疫表型分析、CD4+ T 细胞计数、多重微球分析、高通量筛选以及稀有细胞分析和分选。许多生物分析技术都受到微流控作为分析工具组件的影响，流式细胞术也不例外。在这里，我们详细介绍了现代流式细胞仪的功能和用途，回顾了微流控和微加工设备对流式细胞术领域的近期和历史贡献，考察了当前的应用领域，并提出了将微加工方法与现代流式细胞术协同应用的机会。
