黑龙江立克次体表面蛋白芯片用于远东斑疹热的血清学诊断。
Microarray of surface-exposed proteins of Rickettsia heilongjiangensis for serodiagnosis of Far-eastern spotted fever.
机构信息
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Dia-Jie Street, Fengtai, Beijing, China.
出版信息
BMC Infect Dis. 2014 Jun 17;14:332. doi: 10.1186/1471-2334-14-332.
BACKGROUND
Far-eastern spotted fever (FESF) is an important emerging infectious disease in Northeast Asia. The laboratory diagnosis of FESF in hospitals is mainly based on serological methods. However, these methods need to cultivate rickettsial cells as diagnostic antigens, which is both burdensome and dangerous.
METHODS
Eleven surface-exposed proteins (SEPs) were identified in our previous study and their recombinant proteins (rSEPs) fabricated on a microarray were serologically analyzed with seventeen paired sera from patients suffered from FESF in this study.
RESULTS
All the rSEPs showed sensitivities of between 53% and 82% to acute-phase sera and of between 65% and 82% to convalescent-phase sera, and all the rSEPs except rRplA showed specificities of between 80% and 95%. The combination assay of two, three, or four of the four rSEPs (rOmpA-2, rOmpB-3, rRpsB, and rSdhB) showed better sensitivities of between 76% and 94% to the acute-phase sera or between 82% and 100% to the convalescent-phase sera and acceptable specificities of between 75% and 90%.
CONCLUSIONS
Our results suggest that the four rSEPs are more likely candidate antigens for serological diagnosis of FESF.
背景
远东斑疹热（FESF）是东北亚地区一种重要的新发传染病。医院对 FESF 的实验室诊断主要基于血清学方法。然而，这些方法需要培养立克次体细胞作为诊断抗原，既繁琐又危险。
方法
在我们之前的研究中鉴定了 11 种表面暴露蛋白（SEPs），并在微阵列上制备了它们的重组蛋白（rSEPs），用于本研究中 17 对来自 FESF 患者的急性和恢复期血清进行血清学分析。
结果
所有 rSEPs 对急性期血清的敏感性在 53%至 82%之间，对恢复期血清的敏感性在 65%至 82%之间，除 rRplA 外，所有 rSEPs 的特异性在 80%至 95%之间。四种 rSEPs（rOmpA-2、rOmpB-3、rRpsB 和 rSdhB）中两种、三种或四种的联合检测对急性期血清的敏感性在 76%至 94%之间，对恢复期血清的敏感性在 82%至 100%之间，特异性在 75%至 90%之间。
结论
我们的结果表明，这四种 rSEPs 更有可能成为 FESF 血清学诊断的候选抗原。
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