Department of Preventive Medicine and Biometrics, Uniformed Services University of the Health Sciences, Bethesda, Maryland; Viral and Rickettsial Diseases Department, Infectious Disease Directorate, Naval Medical Research Center, Silver Spring, Maryland; Naval Medical Research Institute Detachment, Lima, Peru.
Am J Trop Med Hyg. 2014 Apr;90(4):690-6. doi: 10.4269/ajtmh.13-0131. Epub 2014 Feb 10.
Reliable laboratory testing is of great importance to detect Bartonella bacilliformis infection. We evaluated the sensitivity and specificity of the enzyme-linked immunosorbent assay (ELISA) using recombinant protein Pap31 (rPap31) for the detection of antibodies against B. bacilliformis as compared with immunofluorescent assay (IFA). Of the 302 sera collected between 1997 and 2000 among an at-risk Peruvian population, 103 and 34 samples tested positive for IFA-immunoglobulin G (IgG) and IFA-IgM, respectively. By using Youden's index, the cutoff values of ELISA-IgG at 0.915 gave a sensitivity of 84.5% and specificity of 94%. The cutoff values of ELISA-IgM at 0.634 gave a sensitivity of 88.2% and specificity of 85.1%. Using latent class analysis, estimates of sensitivity and specificity of almost all the assays were slightly higher than those of a conventional method of calculation. The test is proved beneficial for discriminating between infected and non-infected individuals with the advantage of low-cost and high-throughput capability.
可靠的实验室检测对于检测巴尔通体感染非常重要。我们评估了使用重组蛋白 Pap31（rPap31）的酶联免疫吸附试验（ELISA）检测针对 B. bacilliformis 的抗体的敏感性和特异性，与免疫荧光试验（IFA）相比。在 1997 年至 2000 年间，从秘鲁高危人群中收集了 302 份血清，IFA-免疫球蛋白 G（IgG）和 IFA-IgM 阳性的样本分别为 103 和 34 份。使用约登指数，ELISA-IgG 的截断值为 0.915，敏感性为 84.5%，特异性为 94%。ELISA-IgM 的截断值为 0.634，敏感性为 88.2%，特异性为 85.1%。使用潜在类别分析，几乎所有检测方法的敏感性和特异性估计值均略高于传统计算方法。该检测方法对于区分感染和未感染个体具有成本低、高通量的优势，有助于鉴别。
