Wu Dongping, Chen Xiaoying, Xu Yan, Wang Haiyong, Yu Guangmao, Jiang Luping, Hong Qingxiao, Duan Shiwei
Department of Medical Oncology, Shaoxing People's Hospital, Shaoxing Hospital of Zhejiang University, Zhejiang 312000, P.R. China.
Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
Oncol Lett. 2017 Apr;13(4):2745-2750. doi: 10.3892/ol.2017.5759. Epub 2017 Feb 22.
The DNA mismatch repair (MMR) gene MutL homolog 1 () is critical for the maintenance of genomic integrity. Methylation of the gene promoter was identified as a prognostic marker for numerous types of cancer including glioblastoma, colorectal, ovarian and gastric cancer. The present study aimed to determine whether promoter methylation was associated with survival in male patients with esophageal squamous cell carcinoma (ESCC). Formalin-fixed, paraffin-embedded ESCC tissues were collected from 87 male patients. promoter methylation was assessed using the methylation-specific polymerase chain reaction approach. Kaplan-Meier survival curves and log-rank tests were used to evaluate the association between promoter methylation and overall survival (OS) in patients with ESCC. Cox regression analysis was used to obtain crude and multivariate hazard ratios (HR), and 95% confidence intervals (CI). The present study revealed that promoter methylation was observed in 53/87 (60.9%) of male patients with ESCC. Kaplan-Meier survival analysis demonstrated that promoter hypermethylation was significantly associated with poorer prognosis in patients with ESCC (P=0.048). Multivariate survival analysis revealed that promoter hypermethylation was an independent predictor of poor OS in male patients with ESCC (HR=1.716; 95% CI=1.008-2.921). Therefore, promoter hypermethylation may be a predictor of prognosis in male patients with ESCC.
DNA错配修复（MMR）基因MutL同源物1（）对于维持基因组完整性至关重要。基因启动子的甲基化被确定为包括胶质母细胞瘤、结直肠癌、卵巢癌和胃癌在内的多种癌症的预后标志物。本研究旨在确定启动子甲基化是否与男性食管鳞状细胞癌（ESCC）患者的生存率相关。从87例男性患者中收集福尔马林固定、石蜡包埋的ESCC组织。采用甲基化特异性聚合酶链反应方法评估启动子甲基化。采用Kaplan-Meier生存曲线和对数秩检验评估ESCC患者启动子甲基化与总生存期（OS）之间的关联。采用Cox回归分析获得粗风险比和多变量风险比（HR）以及95%置信区间（CI）。本研究显示，87例男性ESCC患者中有53例（60.9%）检测到启动子甲基化。Kaplan-Meier生存分析表明，启动子高甲基化与ESCC患者预后较差显著相关（P=0.048）。多变量生存分析显示，启动子高甲基化是男性ESCC患者OS不良的独立预测因素（HR=1.716；95%CI=1.008-2.921）。因此，启动子高甲基化可能是男性ESCC患者预后的一个预测指标。
