Chang Microsugar, Huang Yue-Wern, Aronstam Robert S, Lee Han-Jung
Department of Natural Resources and Environmental Studies, National Dong Hwa University, No. 1, Sec. 2, Da-Hsueh Road, Shoufeng Township, Hualien 97401, Taiwan.
Curr Pharm Biotechnol. 2014;15(3):267-75. doi: 10.2174/1389201015666140617095415.
Cellular and nuclear delivery of biomolecules is limited by low membrane permeability. Cell-penetrating peptides (CPPs) can be covalently linked to cargos to improve cellular internalization. Our work indicates that arginine-rich CPPs are also able to interact with a variety of cargos, including DNA, RNA, proteins and nanomaterials, in a noncovalent manner and subsequently effect their delivery into cells. The advantages of noncovalent attachment in CPP-mediated transduction are multiple: ease of use, ease of production, and versatility with respect to both cargo composition and functional delivery (i.e., the cargo is not chemically modified). We have extended this approach to achieve simultaneous transduction of covalently and noncovalently associated complexes, opening a new method for delivering multiple types of cargos, including proteins, fluorescent nanomaterials, nucleic acid and others. These novel variations of CPP-mediated transport should be of broad utility in the transport of genes, small interfering RNAs, proteins and nanoparticles in biomedical research and therapeutic intervention.
生物分子的细胞内和细胞核递送受到低膜通透性的限制。细胞穿透肽（CPPs）可以与货物共价连接以改善细胞内化。我们的工作表明，富含精氨酸的CPPs也能够以非共价方式与多种货物相互作用，包括DNA、RNA、蛋白质和纳米材料，随后将它们递送至细胞中。CPP介导的转导中，非共价连接具有多种优点：使用方便、易于生产，并且在货物组成和功能递送方面具有通用性（即货物未进行化学修饰）。我们扩展了这种方法，以实现共价和非共价相关复合物的同时转导，为递送多种类型的货物（包括蛋白质、荧光纳米材料、核酸等）开辟了一种新方法。CPP介导运输的这些新变体在生物医学研究和治疗干预中的基因、小干扰RNA、蛋白质和纳米颗粒运输中应具有广泛的用途。
