Khandekar Deepa C, Palai Tapas, Agarwal Aman, Bhattacharya Prashant K
Department of Chemical Engineering, Indian Institute of Technology Kanpur, Kanpur, 208016, India.
Bioprocess Biosyst Eng. 2014 Dec;37(12):2529-37. doi: 10.1007/s00449-014-1230-5. Epub 2014 Jun 19.
The study reports the synthesis of fructo-oligosaccharide (FOS) from sucrose using invertase derived from Saccharomyces cerevisiae. The reaction was conducted in a batch mode under free enzyme condition. Fructo-oligosaccharide formation was detected at a high sucrose concentration of over 200 g/L. The investigation was extended to study the effect of different parameters such as initial sucrose concentration (ISC), pH, and enzyme concentration. A maximum FOS yield of 10 % (dry basis) was observed using 525 g/L of ISC, with 6 U/mL of the enzyme, and pH 5.5 at 40 °C. 1-Kestose was the major product of among different forms of FOS. The FOS yield increased with an increase in sucrose concentration up to 525 g/L, beyond which it started to decrease. However, the maximum FOS yield was not affected by the increasing concentration of the enzyme beyond a certain level (2 U/mL). Furthermore, the activity of enzyme slightly increased with an increase in the pH up to 6, and thereafter it declined. Addition of glucose decreased the FOS yield because of enzyme inhibition. A five-step, ten-parameter model was developed, for which the simulation was performed in COPASI. The results predicted by the model were consistent with the experimental data.
该研究报告了利用源自酿酒酵母的转化酶从蔗糖合成低聚果糖（FOS）的过程。反应在游离酶条件下以分批模式进行。在蔗糖浓度高于200 g/L时检测到了低聚果糖的形成。研究范围扩展至研究不同参数的影响，如初始蔗糖浓度（ISC）、pH值和酶浓度。在40°C下，使用525 g/L的ISC、6 U/mL的酶和pH 5.5时，观察到最大低聚果糖产率为10%（干基）。1-蔗果三糖是不同形式低聚果糖中的主要产物。低聚果糖产率随着蔗糖浓度增加至525 g/L而提高，超过该浓度后开始下降。然而，超过一定水平（2 U/mL）后，酶浓度增加对最大低聚果糖产率没有影响。此外，酶活性随着pH值增加至6而略有提高，此后下降。添加葡萄糖由于酶抑制作用而降低了低聚果糖产率。建立了一个五步十参数模型，并在COPASI中进行了模拟。该模型预测的结果与实验数据一致。
