Chaudhary Asma, Hussain Zawar, Ajmal Hafsa, Abdul Rehman Rahat, Abbas Ghulam, Aihetasham Ayesha, Tahira Syeda Anjum
Department of Zoology, Division of Science and Technology, University of Education, Lahore 54770, Pakistan.
Department of Forensic Medicine/Medical Jurisprudence, University of Health Sciences, Lahore 54600, Pakistan.
ACS Omega. 2024 Sep 13;9(38):39652-39662. doi: 10.1021/acsomega.4c04374. eCollection 2024 Sep 24.
The current study focuses on the idea of "Energy from Waste" that intends to address energy crises and manage waste. Fruit waste is one of the most common forms of organic waste due to its inedible portion and perishable nature. In Pakistani regions, an extensive amount of mango pulp (MP)/juice waste is produced due to excessive consumption during summers, which poses huge environmental challenges. The study aims at effective valorization of perishable waste and elimination of deteriorating waste that causes a polluting environment. Experimental work has been conducted to evaluate the sucrolytic potential of FA3 for the bioconversion of sucrose from mango waste into reducing sugars for ethanologenesis. The Plackett-Burman model was designed to analyze enzymatic hydrolytic parameters for sugar conversion. The model was significant for reducing sugars with and values of 43.99 and 0.0013 correspondingly. 11.43 ± 0.068 g/L maximum reducing sugars were analyzed in MP after hydrolysis with 12.58 IU of crude enzyme dosage of FA3 at 30 °C within 5 days with a 22% enzyme conversion rate. Additionally, the ethanologenic potentials of experimental Y34 and standard K7 yeasts were investigated from mango hydrolyzate when subjected to central composite design as a statistical optimization tool. These findings exhibited significantly higher response outcomes and good development for waste management.
当前的研究聚焦于“垃圾能源”这一理念，旨在解决能源危机并管理垃圾。水果垃圾因其不可食用部分和易腐性质，是最常见的有机垃圾形式之一。在巴基斯坦地区，由于夏季过度消费，产生了大量芒果果肉（MP）/果汁垃圾，这带来了巨大的环境挑战。该研究旨在实现易腐垃圾的有效增值，并消除造成环境污染的变质垃圾。已开展实验工作，以评估FA3将芒果垃圾中的蔗糖生物转化为用于乙醇发酵的还原糖的蔗糖分解潜力。采用Plackett-Burman模型分析糖转化的酶水解参数。该模型对还原糖具有显著意义，其R²和p值分别为43.99和0.0013。在30℃下，用12.58 IU的FA3粗酶剂量在5天内对MP进行水解后，分析得到最大还原糖含量为11.43±0.068 g/L，酶转化率为22%。此外，当采用中心复合设计作为统计优化工具时，研究了实验用Y34酵母和标准K7酵母从芒果水解物中的乙醇发酵潜力。这些发现显示出显著更高的响应结果以及在垃圾管理方面的良好进展。
