Department of Clinical Sciences, University of Central Florida College of Medicine, Orlando, Florida.
Cancer Cytopathol. 2014 Sep;122(9):639-45. doi: 10.1002/cncy.21451. Epub 2014 Jun 17.
Nucleic acid-based high-risk human papillomavirus (hrHPV) testing is essential to contemporary cervical cancer screening. The numbers of commercially available assays approved by the US Food and Drug Administration for HPV nucleic acid detection have increased, each offering various approaches to analysis. An understanding of the methodologies associated with HPV testing is important to the practice of laboratory medicine. An overview of instruments, chemistries, laboratory workflows, and test limitations associated with current US Food and Drug Administration-approved assays is provided.
基于核酸的高危型人乳头瘤病毒（hrHPV）检测对于当代宫颈癌筛查至关重要。美国食品和药物管理局批准用于 HPV 核酸检测的商业检测试剂盒数量不断增加，每种试剂盒都提供了不同的分析方法。了解 HPV 检测相关的方法学对于实验室医学实践非常重要。本文概述了当前获得美国食品和药物管理局批准的检测试剂盒所涉及的仪器、化学、实验室工作流程以及检测局限性。
