Hamada Kazuhide, Miura Yoichi, Toma Naoki, Miyamoto Keiichi, Imanaka-Yoshida Kyoko, Matsushima Satoshi, Yoshida Toshimichi, Taki Waro, Suzuki Hidenori
Department of Neurosurgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie, 514-8507, Japan.
Transl Stroke Res. 2014 Oct;5(5):595-603. doi: 10.1007/s12975-014-0352-z. Epub 2014 Jun 19.
The aims of this study were to develop a new coil, gellan sulfate core platinum coil (GSCC), that delivers tenascin-C (TNC) to an aneurysm (GSCC-TNC) and to evaluate the effects on intra-aneurysmal organization. We performed in vitro adsorption tests of TNC to gellan sulfate (GS). GSCC-TNC was produced by immersing GSCC in TNC solution under the following conditions (TNC concentration 10, 50, or 100 μg/mL; TNC immersion time 15, 30, or 60 min) by monitoring intra-aneurysmal organization in a rat blind-ended aneurysm model. In addition, 20 rats randomly underwent implantation of a platinum coil or the GSCC-TNC produced under optimum conditions into an aneurysm, whose organization effects were compared in a blind fashion at 2 weeks post-surgery. GS demonstrated a high affinity to TNC in a dose-dependent fashion (affinity constant = 1.79 × 10(10) (M(-1))). GSCC immersed in 10 μg/mL of TNC solution for 30 and 60 min induced similar and better organization of aneurysmal cavity compared with that for 15 min (the ratio of the organized areas in an aneurysmal cavity-15 min, 27.2 ± 11.8 %; 30 min, 75.6 ± 11.9 %; 60 min, 82.6 ± 19.7 %, respectively) with the preservation of the aneurysmal wall structure, while higher TNC concentrations caused the destruction of the aneurysmal wall. GSCC-TNC produced under 10 μg/mL of TNC solution for 30 min showed a significantly better organization of aneurysms compared with bare platinum coils in rats. A newly developed coil, GSCC-TNC, may be effective for improving intra-aneurysmal organization after coil embolization.
本研究的目的是研发一种新型线圈——硫酸结冷胶核心铂线圈（GSCC），其可将腱生蛋白-C（TNC）递送至动脉瘤（GSCC-TNC），并评估其对动脉瘤腔内组织化的影响。我们进行了TNC对硫酸结冷胶（GS）的体外吸附试验。通过在大鼠盲端动脉瘤模型中监测动脉瘤腔内组织化情况，在以下条件下（TNC浓度10、50或100μg/mL；TNC浸泡时间15、30或60分钟）将GSCC浸入TNC溶液中来制备GSCC-TNC。此外，20只大鼠被随机植入铂线圈或在最佳条件下制备的GSCC-TNC至动脉瘤中，术后2周以盲法比较其组织化效果。GS对TNC表现出剂量依赖性的高亲和力（亲和常数=1.79×10(10)（M(-1)））。与浸泡15分钟相比，将GSCC浸入10μg/mL的TNC溶液中30分钟和60分钟可诱导动脉瘤腔形成相似且更好的组织化（动脉瘤腔内组织化区域的比例——15分钟时为27.2±11.8%；30分钟时为75.6±11.9%；60分钟时为82.6±19.7%），同时保留动脉瘤壁结构，而较高的TNC浓度会导致动脉瘤壁破坏。在大鼠中，在10μg/mL的TNC溶液中制备30分钟的GSCC-TNC与裸铂线圈相比，对动脉瘤的组织化效果明显更好。新研发的线圈GSCC-TNC可能对改善线圈栓塞术后的动脉瘤腔内组织化有效。
