Matsumoto Hiroyuki, Terada Tomoaki, Tsuura Mitsuharu, Itakura Toru, Ogawa Atsushi
Department of Neurological Surgery, Wakayama Medical University, Wakayama, Japan.
Neurosurgery. 2003 Aug;53(2):402-7; discussion 407-8. doi: 10.1227/01.neu.0000073728.82721.8e.
We developed a new type of platinum coil that has a polyvinyl alcohol (PVA) core to absorb and release various biologically active materials for the endovascular treatment of intracranial aneurysms. We evaluated its efficacy as an embolic material with basic fibroblast growth factor (bFGF) in an in vitro and in vivo study.
A small PVA thread was inserted into the central space of the primary coil. This coil was named the PVA-core coil. Ten-millimeter segments of the PVA-core coil were used in this study. PVA-core coils were immersed in a bFGF solution for 1 hour. The PVA-core coil, which absorbed bFGF in the PVA-core, was named the FGF-core coil. Initially, the dose of bFGF released from the PVA core into saline was measured by performing an enzyme-linked immunosorbent assay. In the in vitro study, FGF-core coils, PVA-core coils, and unmodified coils were cultured with fibroblasts (NIH3T3), and then their surfaces were observed with electron microscopy. In the in vivo study, each coil was implanted into a rat common carotid artery. The rats were killed, and the arterial lumen was histologically examined at 14 and 28 days after coil implantation.
BFGF was released from the PVA core into saline within 24 hours by performing an enzyme-linked immunosorbent assay. Electron microscopic findings demonstrated remarkable cellular adhesion to the surfaces of the FGF-core coils, but no adhesion to the surfaces of the PVA-core coils and the unmodified coils was found. Histologically, remarkable cell proliferation in the vascular lumen was demonstrated in the common carotid arteries of the FGF-core coil implantation group at the 14th and 28th days. Cellular components proliferated around the implanted coil, and these components mainly stained blue with Masson trichrome. These changes did not occur in the PVA-core coil group and the unmodified coil group.
We suggest that FGF-core coils may be effective in inducing fibrotic changes inside aneurysms. These coils may be used as an embolic material to cure cerebral aneurysms.
我们研发了一种新型铂线圈，其具有聚乙烯醇（PVA）核心，可吸收和释放各种生物活性物质，用于颅内动脉瘤的血管内治疗。在一项体外和体内研究中，我们评估了其作为含碱性成纤维细胞生长因子（bFGF）的栓塞材料的疗效。
将一根细小的PVA线插入初级线圈的中心空间。该线圈被命名为PVA核心线圈。本研究使用10毫米长的PVA核心线圈段。将PVA核心线圈浸入bFGF溶液中1小时。在PVA核心中吸收了bFGF的PVA核心线圈被命名为FGF核心线圈。最初，通过酶联免疫吸附测定法测量从PVA核心释放到盐水中的bFGF剂量。在体外研究中，将FGF核心线圈、PVA核心线圈和未修饰的线圈与成纤维细胞（NIH3T3）一起培养，然后用电子显微镜观察它们的表面。在体内研究中，将每个线圈植入大鼠颈总动脉。在植入线圈后14天和28天处死大鼠，并对动脉腔进行组织学检查。
通过酶联免疫吸附测定法，bFGF在24小时内从PVA核心释放到盐水中。电子显微镜检查结果显示，FGF核心线圈表面有明显的细胞粘附，但未发现PVA核心线圈和未修饰线圈表面有粘附现象。组织学检查显示，在第14天和第28天，FGF核心线圈植入组的颈总动脉血管腔内有明显的细胞增殖。植入线圈周围的细胞成分增殖，这些成分在用马松三色染色时主要染成蓝色。这些变化在PVA核心线圈组和未修饰线圈组中未出现。
我们认为FGF核心线圈可能在诱导动脉瘤内纤维化改变方面有效。这些线圈可用作治疗脑动脉瘤的栓塞材料。
