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在一种杆状病毒的外壳蛋白中赖氨酸乙酰化的证据。

Evidence for lysine acetylation in the coat protein of a polerovirus.

机构信息

Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA.

Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.

出版信息

J Gen Virol. 2014 Oct;95(Pt 10):2321-2327. doi: 10.1099/vir.0.066514-0. Epub 2014 Jun 17.

DOI:10.1099/vir.0.066514-0
PMID:24939649
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4165934/
Abstract

Virions of the RPV strain of Cereal yellow dwarf virus-RPV were purified from infected oat tissue and analysed by MS. Two conserved residues, K147 and K181, in the virus coat protein, were confidently identified to contain epsilon-N-acetyl groups. While no functional data are available for K147, K181 lies within an interfacial region critical for virion assembly and stability. The signature immonium ion at m/z 126.0919 demonstrated the presence of N-acetyllysine, and the sequence fragment ions enabled an unambiguous assignment of the epsilon-N-acetyl modification on K181. We hypothesize that selection favours acetylation of K181 in a fraction of coat protein monomers to stabilize the capsid by promoting intermonomer salt bridge formation.

摘要

从感染的燕麦组织中纯化了 RPV 株的 Cereal yellow dwarf virus-RPV 病毒粒子，并通过 MS 进行了分析。在病毒外壳蛋白中鉴定出两个保守的残基 K147 和 K181，它们都含有 ε-N-乙酰基。虽然 K147 没有功能数据，但 K181 位于对病毒粒子组装和稳定性至关重要的界面区域。质荷比 m/z 126.0919 处的特征免疫离子表明存在 N-乙酰赖氨酸，序列片段离子能够明确 K181 上的 ε-N-乙酰化修饰。我们假设，选择有利于部分外壳蛋白单体上的 K181 乙酰化，通过促进单体间盐桥形成来稳定衣壳。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1ae/4165934/2aec0c954064/066514-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1ae/4165934/73c05201c137/066514-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1ae/4165934/3697ae0a6ef3/066514-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1ae/4165934/2aec0c954064/066514-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1ae/4165934/73c05201c137/066514-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1ae/4165934/3697ae0a6ef3/066514-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1ae/4165934/2aec0c954064/066514-f3.jpg

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