Fehér Enikő, Lengyel György, Dán Adám, Farkas Szilvia L, Bányai Krisztián
Hungarian Academy of Sciences Institute for Veterinary Medical Research, Centre for Agricultural Research Budapest Hungary.
Hungarian Defence Forces Military Medical Centre Budapest Hungary.
Acta Microbiol Immunol Hung. 2014 Jun;61(2):221-7. doi: 10.1556/AMicr.61.2014.2.11.
Goose haemorrhagic polyomavirus (GHPV) provoke haemorrhagic nephritis and enteritis of domestic geese. Outbreaks were detected in European countries and caused economic losses for goose keepers. Domestic ducks may be infected with GHPV without any signs typical for geese. The genomic organisation of some isolates was described but the gene functions and the pathomechanisms of the virus was not precisely defined. Here we describe the genome sequence and structure of GHPV of a goose from a Hungarian goose flock showing characteristics of the haemorrhagic nephritis and enteritis. The GHPV genome investigated in this study was 5252 bp long and was very similar (99% nucleotide identity) to sequences deposited in the GenBank. All the whole GHPV genomes possess the same ORFs in length, including the VP1, VP2, VP3, ORF-X, t and T tumour antigens. Amino acid changes are detected mainly in the putative ORF-X region. Data about the GHPV genome imply a conserved genomic structure among isolates from different countries. Genomic and epidemiological studies may help vaccine development efforts and identify potential heterologous reservoirs of GHPV.
鹅出血性多瘤病毒（GHPV）可引发家鹅的出血性肾炎和肠炎。欧洲国家已检测到疫情爆发，给养鹅户造成了经济损失。家鸭可能感染GHPV，但无任何典型的鹅类症状。已描述了一些分离株的基因组结构，但该病毒的基因功能和致病机制尚未明确界定。在此，我们描述了一只来自匈牙利鹅群、表现出出血性肾炎和肠炎特征的鹅的GHPV基因组序列和结构。本研究中所研究的GHPV基因组长度为5252 bp，与GenBank中 deposited 的序列非常相似（核苷酸同一性为99%）。所有完整的GHPV基因组在长度上具有相同的开放阅读框，包括VP1、VP2、VP3、ORF-X、t和T肿瘤抗原。氨基酸变化主要在假定的ORF-X区域被检测到。关于GHPV基因组的数据表明，来自不同国家的分离株之间存在保守的基因组结构。基因组和流行病学研究可能有助于疫苗研发工作，并识别GHPV的潜在异源宿主。
