[通过生物发光法测定生物胺]
[Assay of biogenic amines by bioluminescence].
作者信息
Fraysse M, Dumas P, Nicot G, Lachâtre G, Habrioux G
机构信息
Laboratoire de Biochimie, Faculté de Pharmacie, Limoges.
出版信息
C R Acad Sci III. 1989;308(7):195-8.
A bioluminescent procedure to measure noradrenaline and serotonin has been realized. The amines are oxidized by the monoamine oxidase of pig brain mitochondria. The NH3 generated in this reaction is directly measured by enzymatic reaction. The coenzyme of this last reaction is the NADH,H+ which is measured with a bioluminescent system: the FMN-oxidoreductase-luciferase. The extension to other amines is possible, it depends only of the specificity of the monoamine oxidase.
已实现一种用于测量去甲肾上腺素和血清素的生物发光方法。这些胺类被猪脑线粒体的单胺氧化酶氧化。该反应中产生的NH₃通过酶促反应直接测量。最后这个反应的辅酶是NADH、H⁺，它通过生物发光系统进行测量：FMN氧化还原酶 - 荧光素酶。将该方法扩展到其他胺类是可行的，这仅取决于单胺氧化酶的特异性。
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