Angeles-Shim Rosalyn B, Vinarao Ricky B, Marathi Balram, Jena Kshirod K
From the Novel Gene Resources Laboratory, Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines (Angeles-Shim, Vinarao, Marathi, and Jena); and the Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, Aichi 464-8601, Japan (Angeles-Shim).
J Hered. 2014 Sep-Oct;105(5):676-89. doi: 10.1093/jhered/esu032. Epub 2014 Jun 17.
Oryza latifolia is a tetraploid wild Oryza species with a CCDD genome that has been reported to harbor resistance to bacterial blight (BB), brown planthopper, and whitebacked planthopper. Aside from these traits, O. latifolia is also being tapped as a new source of resistance to lodging and high biomass production. To explore the genetic potential of O. latifolia as a novel genetic resource for the improvement of existing O. sativa cultivars, 27 disomic derivatives of O. latifolia monosomic alien addition lines (MAAL) were characterized for alien chromosome segment introgressions and evaluated for yield components, BB resistance, and strong stem characteristics. A total of 167 simple sequence repeat, sequence tagged site, and single nucleotide polymorphism markers, along with newly developed indel markers that were specifically designed to detect O. latifolia chromosome segment introgressions in an O. sativa background, were used to define alien introgressions in 27 disomics derived from O. latifolia MAALs. Genotype data showed that 32 unique introgressions spanning 0.31-22.73 Mb were introgressed in different combinations in each of the 27 disomic derivatives. Evaluation of the disomic derivatives for agronomic traits identified lines with putative QTLs for resistance to Philippine races 3A, 4, 9A, and 9D of BB. Putative quantitative trait loci (QTLs) conferring strong stem in 19 out of the 27 disomic derivatives studied were also identified from O. latifolia introgressions on chromosome 6.
宽叶野生稻是一种具有CCDD基因组的四倍体野生稻种，据报道它对水稻白叶枯病、褐飞虱和白背飞虱具有抗性。除了这些特性外，宽叶野生稻还被开发为抗倒伏和高生物量生产的新抗性来源。为了探索宽叶野生稻作为改良现有栽培稻品种的新型遗传资源的遗传潜力，对27个宽叶野生稻单体异附加系（MAAL）的二体衍生物进行了外源染色体片段渗入特征分析，并对产量构成因素、白叶枯病抗性和茎秆粗壮特性进行了评估。总共使用了167个简单序列重复、序列标签位点和单核苷酸多态性标记，以及专门设计用于检测栽培稻背景下宽叶野生稻染色体片段渗入的新开发的插入缺失标记，来确定源自宽叶野生稻MAALs的27个二体中的外源渗入情况。基因型数据显示，在27个二体衍生物中的每一个中，有32个独特的渗入片段，跨度为0.31-22.73 Mb，以不同组合渗入。对二体衍生物的农艺性状评估确定了对白叶枯病菲律宾小种3A、4、9A和9D具有抗性的假定QTL的品系。在研究的27个二体衍生物中的19个中，还从6号染色体上的宽叶野生稻渗入片段中鉴定出了赋予茎秆粗壮的假定数量性状位点（QTL）。
