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番茄属（L.）与（加缪）劳纳特属（A. Camus）之间属间杂种的培育。

Development of an intergeneric hybrid between L. and (A. Camus) Launert.

作者信息

Ballesfin Ma LaRue E, Vinarao Ricky B, Sapin Janice, Kim Sung-Ryul, Jena Kshirod K

机构信息

Novel Gene Resources Laboratory, Strategic Innovation Platform, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines.

出版信息

Breed Sci. 2018 Sep;68(4):474-480. doi: 10.1270/jsbbs.18045. Epub 2018 Aug 23.

DOI:10.1270/jsbbs.18045
PMID:30369822
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6198897/
Abstract

An intergeneric hybrid was successfully developed between L. (IRRI 154) and (A. Camus) Launert using embryo rescue technique in this study. A low crossability value (0.07%) implied that there was high incompatibility between the two species of the hybrid. The F hybrid showed intermediate phenotypic characteristics between the parents but the plant height was very short. The erect plant type resembled the female parent IRRI 154 but the leaves were similar to . Cytological analysis revealed highly non-homology between chromosomes of the two species as the F plants showed 24 univalents without any chromosome pairing. The F hybrid plant was further confirmed by PCR analysis using the newly designed 11 indel markers showing polymorphism between and . This intergeneric hybrid will open up opportunities to transfer novel valuable traits from into cultivated rice.

摘要

本研究利用胚挽救技术成功培育出了一种李氏禾（IRRI 154）与假稻（加缪假稻）劳纳特之间的属间杂种。较低的杂交亲和性值（0.07%）表明这两个杂交物种之间存在高度不亲和性。F1杂种在表型特征上介于双亲之间，但株高非常矮。直立的株型类似于母本IRRI 154，但叶片与假稻相似。细胞学分析表明，这两个物种的染色体之间高度不同源，因为F1植株显示出24个单价体，没有任何染色体配对。通过使用新设计的11个在李氏禾和假稻之间表现出多态性的插入缺失标记进行PCR分析，进一步证实了该F1杂种植株。这种属间杂种将为把假稻的新的有价值性状转移到栽培稻中提供机会。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e98/6198897/edc7cad84d19/68_18045_5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e98/6198897/0a7132a48cab/68_18045_1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e98/6198897/dca0ef3f85a1/68_18045_2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e98/6198897/c598d3d9c63e/68_18045_3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e98/6198897/3352443e35a0/68_18045_4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e98/6198897/edc7cad84d19/68_18045_5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e98/6198897/0a7132a48cab/68_18045_1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e98/6198897/dca0ef3f85a1/68_18045_2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e98/6198897/c598d3d9c63e/68_18045_3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e98/6198897/3352443e35a0/68_18045_4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e98/6198897/edc7cad84d19/68_18045_5.jpg

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