克氏原螯虾肝胰腺和绿腺微粒体混合功能氧化酶系统的特性研究

Characterization of the microsomal mixed-function oxygenase system of the hepatopancreas and green gland of the red swamp crayfish, Procambarus clarkii.

Jewell C S, Winston G W

Institute of Environmental Studies, Louisiana State University, Baton Rouge 70803.

Comp Biochem Physiol B. 1989;92(2):329-39. doi: 10.1016/0305-0491(89)90287-3.

Abstract

The microsomal mixed-function oxygenase (MFO) system from the hepatopancreas and green gland of the red swamp crayfish, Procambarus clarkii has been characterized with respect to the constitution of electron transport proteins and the ability to catalyze the metabolism of xenobiotics. 2. Cytochrome P-450 content of hepatopancreas microsomes was approximately 10-fold higher than that of green gland and comparable to that of rat liver. NADPH-cytochrome c reductase activity in hepatopancreas microsomes was approximately 2% of that found in rat liver microsomes. 3. Green gland microsomes catalyzed higher turnover rates of aminopyrine N-demethylase and benzo[a]pyrene hydroxylase than hepatopancreas microsomes. With hepatopancreas microsomes, organic hydroperoxides supported a greater rate of aminopyrine N-demethylation than did NADPH plus O2. 4. P. clarkii hepatopancreas microsomes generally displayed a lower binding affinity (Ks) for a number of type I and type II ligands than did rat liver microsomes.

摘要

对克氏原螯虾肝胰腺和绿腺中的微粒体混合功能氧化酶（MFO）系统在电子传递蛋白组成及催化异生物质代谢能力方面进行了表征。2. 肝胰腺微粒体中的细胞色素P-450含量比绿腺中的高约10倍，与大鼠肝脏中的相当。肝胰腺微粒体中NADPH-细胞色素c还原酶活性约为大鼠肝脏微粒体中该酶活性的2%。3. 绿腺微粒体催化氨基比林N-脱甲基酶和苯并[a]芘羟化酶的周转速率高于肝胰腺微粒体。对于肝胰腺微粒体，有机氢过氧化物比NADPH加O2支持更高的氨基比林N-脱甲基速率。4. 与大鼠肝脏微粒体相比，克氏原螯虾肝胰腺微粒体对多种I型和II型配体通常表现出较低的结合亲和力（Ks）。