Zheng Li, Yang Jinsong, Fan Hongsong, Zhang Xingdong
Medical and Scientific Research Center, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
Osteopathy Ward of The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
Exp Ther Med. 2014 May;7(5):1147-1150. doi: 10.3892/etm.2014.1583. Epub 2014 Feb 25.
Certain materials may mimic natural cartilage to provide an amenable cellular microenvironment for the chondrogenic differentiation of mesenchymal stem cells. The chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) has been demonstrated to be induced by collagen-based hydrogels , but whether the induction is material-driven or self-differentiation has not been elucidated. In the present study, BMSCs were encapsulated in porous materials, namely, a biphasic calcium phosphate ceramic (BCP), silk fibroin protein matrix (SFP) and collagen sponge (CS), to further study the chondrogenic effects of various materials. Diffusion chambers that allow the body fluid to permeate and deter the host cells from invasion were also loaded with the cell-scaffold constructs. Chambers containing the scaffold-BMSC composites were implanted subcutaneously in the dorsa of rabbits. The specimens in the chamber were harvested for histological and immunohistochemical analyses eight weeks after implantation. The results showed that no chondrogenic differentiation of the BMSCs occurred when the BMSCs were encapsulated in BCP, SFP and CS, indicating that chondrogenesis induced by materials is material-dependent and that these particular porous materials are not suitable for inducing chondrogenesis. However, the diffusion chamber was effective in preventing host immune rejection, host cell invasion and vascular invasion. The results are likely to serve as a valuable clinical reference when selecting an appropriate scaffold for cartilage repair.
某些材料可模拟天然软骨，为间充质干细胞的软骨分化提供适宜的细胞微环境。骨髓间充质干细胞（BMSCs）的软骨分化已被证明可由胶原基水凝胶诱导，但这种诱导是材料驱动还是自我分化尚未阐明。在本研究中，将BMSCs封装在多孔材料中，即双相磷酸钙陶瓷（BCP）、丝素蛋白基质（SFP）和胶原海绵（CS）中，以进一步研究各种材料的软骨生成作用。还将允许体液渗透并阻止宿主细胞侵入的扩散小室加载细胞支架构建体。将含有支架 - BMSCs复合材料的小室皮下植入兔背部。植入八周后，从小室中取出标本进行组织学和免疫组织化学分析。结果表明，当BMSCs封装在BCP、SFP和CS中时，未发生BMSCs的软骨分化，这表明材料诱导的软骨生成是材料依赖性的，并且这些特定的多孔材料不适合诱导软骨生成。然而，扩散小室在防止宿主免疫排斥、宿主细胞侵入和血管侵入方面是有效的。这些结果在选择合适的软骨修复支架时可能作为有价值的临床参考。
