Fujisato T, Sajiki T, Liu Q, Ikada Y
Research Center for Biomedical Engineering, Kyoto University, Japan.
Biomaterials. 1996 Jan;17(2):155-62. doi: 10.1016/0142-9612(96)85760-7.
A chondrocyte-collagen composite was prepared in an attempt to regenerate cartilage by its subcutaneous implantation in nude mouse. When the composite was impregnated with basic fibroblast growth factor (bFGF) prior to implantation, regeneration of the cartilage tissue was remarkably accelerated. Histological staining of the implanted composites with Safranin O-fast green revealed that the cells incorporated in the composites exhibited their phenotype and formed a new matured cartilage. A thin layer of fibrous capsule was observed surrounding the implanted composite and the inflammatory response of the host to the implant was mild. Specific proteoglycans were accumulated in the composite even 1 week after implantation. At 2 weeks after implantation, the chondrocytes regenerated the cartilage tissue, although still immature, but at 4 weeks almost all of the chondrocytes transferred to the mature stage. Conversely, such mature cartilage tissue was not noticed up to 4 weeks after implantation if the collagen scaffold was not impregnated with bFGF. Moreover, the mature area was limited to only a small fraction of the implanted composite, unless bFGF was incorporated in it.
制备了软骨细胞 - 胶原蛋白复合物，试图通过将其皮下植入裸鼠体内来再生软骨。当复合物在植入前用碱性成纤维细胞生长因子（bFGF）浸渍时，软骨组织的再生明显加速。用番红O - 固绿对植入的复合物进行组织学染色显示，复合物中包含的细胞表现出其表型并形成了新的成熟软骨。观察到植入的复合物周围有一层薄的纤维囊，宿主对植入物的炎症反应较轻。即使在植入1周后，特异性蛋白聚糖仍在复合物中积累。植入后2周，软骨细胞再生了软骨组织，尽管仍不成熟，但在4周时几乎所有软骨细胞都进入了成熟阶段。相反，如果胶原蛋白支架未用bFGF浸渍，则在植入后4周内未观察到这种成熟的软骨组织。此外，除非在其中加入bFGF，否则成熟区域仅限于植入复合物的一小部分。
