Ran Feng, Liu Changjian, Liu Zhao, Shang Tao, Zhou Min, Qiao Tong
Department of Vascular Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China.
Exp Ther Med. 2014 May;7(5):1193-1196. doi: 10.3892/etm.2014.1562. Epub 2014 Feb 19.
The aim of the present study was to investigate whether chronic administration of basic fibroblast growth factor (bFGF) following angioplasty in a dog model of atherosclerotic iliac stenosis may restore endothelium function and prevent restenosis (RS). In total, 40 dogs with atherosclerotic stenosis of the right iliac arteries were used in the study. A total of 20 dogs underwent histological examination of the lumen areas prior to (n=10) and immediately following angioplasty (n=10). Intravenous bFGF was administered to 10 dogs (bFGF group) and an additional 10 dogs received vehicle injection (control group). Animals in the two groups were sacrificed 42 days following surgery for analysis of vascular reactivity and morphometric assessment of the histological cross-sectional areas. The bFGF group exhibited significantly greater maximal endothelium-dependent acetylcholine-induced relaxation (E, 43±9%) when compared with the control group (E, 8±6%; P<0.05). In addition, the maximal endothelium-independent response of the bFGF group to sodium nitroprusside (E, 90±2%) was greater than that of the control group (E, 60±2%; P<0.05). Six weeks following angioplasty, the lumen area in the bFGF group (2.01±0.78 mm) was greater compared with the control group (1.0±0.10%). The lumen area decreased by 58% between immediately after angioplasty and the control group six weeks following angioplasty. Therefore, the results of the present study indicated that administration of bFGF may not only restore endothelium-dependent and -independent relaxation, but also prevent RS in dogs that have undergone angioplasty.
本研究的目的是调查在动脉粥样硬化性髂动脉狭窄犬模型中，血管成形术后长期给予碱性成纤维细胞生长因子（bFGF）是否可恢复内皮功能并预防再狭窄（RS）。本研究共使用了40只患有右侧髂动脉粥样硬化狭窄的犬。共有20只犬在血管成形术前（n = 10）和血管成形术后立即（n = 10）接受了管腔区域的组织学检查。10只犬静脉注射bFGF（bFGF组），另外10只犬接受载体注射（对照组）。两组动物在术后42天处死，用于分析血管反应性和组织学横截面积的形态学评估。与对照组（E，8±6%；P<0.05）相比，bFGF组内皮依赖性乙酰胆碱诱导的最大舒张（E，43±9%）显著更大。此外，bFGF组对硝普钠的最大非内皮依赖性反应（E，90±2%）大于对照组（E，60±2%；P<0.05）。血管成形术后六周，bFGF组的管腔面积（2.01±0.78 mm）大于对照组（1.0±0.10%）。血管成形术后即刻至六周后对照组之间，管腔面积减少了58%。因此，本研究结果表明，给予bFGF不仅可恢复内皮依赖性和非依赖性舒张，还可预防接受血管成形术犬的再狭窄。
