暴露于人类体外受精取卵条件下的小鼠卵母细胞的受精与卵裂

Fertilization and cleavage of mouse oocytes exposed to the conditions of human oocyte retrieval for in vitro fertilization.

作者信息

Jackson K V, Kiessling A A

机构信息

Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115.

出版信息

Fertil Steril. 1989 Apr;51(4):675-81. doi: 10.1016/s0015-0282(16)60620-9.

DOI:10.1016/s0015-0282(16)60620-9

PMID:2494083

Abstract

Ova from two strains of mice (a hybrid-inbred strain, B6D2F1, and a random-bred strain, CD1) were shocked by exposure to environmental conditions possibly encountered by human oocytes retrieved for in vitro fertilization (IVF). Shocked and control mouse ova were fertilized in vitro in either simple or complex media and zygote development to morulae and blastocyst stages compared with that of zygotes fertilized in vivo. Development of the hybrid-inbred zygotes following fertilization in the simple media of shocked and control ova was essentially the same as for ova fertilized in vivo (84 +/- 6.6, 89 +/- 1.6, 87 +/- 6.0% to the 2-cell stage and 89 +/- 5.2, 94 +/- 2.3, 99 +/- 1.0% of two cells to blastocysts, respectively); development in the complex media also was the same following fertilization of shocked and control ova (80 +/- 8.7, 90 +/- 2.7% to two cells and 33 +/- 3.5, 35 +/- 4.5% of two cells to blastocysts, respectively) but lower than that of in vivo zygotes (92 +/- 4.6 to two cells, 58 +/- 4.7 two cells to blastocysts). In contrast, the fertilization and development in the simple media of shocked and control random-bred ova was lower and more variable (90 +/- 5.8, 67 +/- 11.1% to two cells and 17 +/- 8.3, 42 +/- 13.6% two cells to blastocysts, respectively) than the development of in vivo zygotes (96 +/- 1.5% to two cells, 51 +/- 5.5 two cells to blastocysts).(ABSTRACT TRUNCATED AT 250 WORDS)

摘要

将来自两种品系小鼠（一种杂交近交系B6D2F1和一种随机繁殖系CD1）的卵母细胞暴露于人类体外受精（IVF）所获取的卵母细胞可能遇到的环境条件下进行冲击。将经过冲击和未冲击的小鼠卵母细胞分别在简单或复杂培养基中进行体外受精，并将受精卵发育至桑椹胚和囊胚阶段，与体内受精的受精卵进行比较。在简单培养基中，经过冲击和未冲击的近交系杂种受精卵受精后的发育情况与体内受精的卵母细胞基本相同（分别为84±6.6%、89±1.6%、87±6.0%发育至2细胞阶段，以及89±5.2%、94±2.3%、99±1.0%的2细胞发育至囊胚）；在复杂培养基中，经过冲击和未冲击的卵母细胞受精后的发育情况也相同（分别为80±8.7%、90±2.7%发育至2细胞，以及33±3.5%、35±4.5%的2细胞发育至囊胚），但低于体内受精卵（92±4.6%发育至2细胞，58±4.7%的2细胞发育至囊胚）。相比之下，在简单培养基中，经过冲击和未冲击的随机繁殖系卵母细胞的受精和发育情况低于体内受精卵且变异性更大（分别为90±5.8%、67±11.1%发育至2细胞，以及17±8.3%、42±13.6%的2细胞发育至囊胚），体内受精卵的发育情况为（96±1.5%发育至2细胞，51±5.5%的2细胞发育至囊胚）。（摘要截选至250词）