显微注射反义c-mos寡核苷酸可阻止成熟小鼠卵母细胞进入减数分裂II期。
Microinjection of antisense c-mos oligonucleotides prevents meiosis II in the maturing mouse egg.
作者信息
O'Keefe S J, Wolfes H, Kiessling A A, Cooper G M
机构信息
Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115.
出版信息
Proc Natl Acad Sci U S A. 1989 Sep;86(18):7038-42. doi: 10.1073/pnas.86.18.7038.
Injection of antisense oligonucleotides was used to investigate the function of c-mos in murine oocytes. Oocytes injected with antisense c-mos oligonucleotides completed the first meiotic division but failed to initiate meiosis II. Instead, loss of c-mos function led to chromosome decondensation, reformation of a nucleus after meiosis I, and cleavage to two cells. Therefore, c-mos is required for meiosis II during murine oocyte maturation.
注射反义寡核苷酸用于研究c-mos在小鼠卵母细胞中的功能。注射了反义c-mos寡核苷酸的卵母细胞完成了第一次减数分裂，但未能启动减数分裂II。相反，c-mos功能的丧失导致染色体解聚、减数分裂I后细胞核重新形成，并分裂为两个细胞。因此，c-mos是小鼠卵母细胞成熟过程中减数分裂II所必需的。
Zotero 插件