Takasu N, Yamada T, Shimizu Y, Nagasawa Y, Komiya I
Department of Gerontology, Endocrinology and Metabolism, School of Medicine, Shinshu University, Nagano-ken, Japan.
J Endocrinol. 1989 Mar;120(3):503-8. doi: 10.1677/joe.0.1200503.
This study set out to elucidate the mechanism by which H2O2 generation is regulated in cultured porcine thyroid cells. We monitored continuously the effects of the calcium ionophore A23187 and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) on H2O2 generation, using homovanillic acid and horseradish peroxidase. A23187 and TPA stimulated H2O2 generation. A23187 increased cytoplasmic free calcium and TPA activated protein kinase C. Generation of H2O2 is therefore regulated by cytoplasmic free calcium and protein kinase C. Exposure to A23187 or TPA augmented further the stimulation of H2O2 generation by TPA or A23187 respectively. Thus A23187 and TPA, by increasing cytoplasmic free calcium and activating protein kinase C respectively, synergistically activate H2O2 generation.
本研究旨在阐明培养的猪甲状腺细胞中过氧化氢（H2O2）生成的调控机制。我们使用高香草酸和辣根过氧化物酶，持续监测钙离子载体A23187和佛波酯12-O-十四酰佛波醇-13-乙酸酯（TPA）对H2O2生成的影响。A23187和TPA刺激了H2O2的生成。A23187增加了细胞质游离钙，TPA激活了蛋白激酶C。因此，H2O2的生成受细胞质游离钙和蛋白激酶C的调控。暴露于A23187或TPA分别进一步增强了TPA或A23187对H2O2生成的刺激作用。因此，A23187和TPA分别通过增加细胞质游离钙和激活蛋白激酶C，协同激活H2O2的生成。
