Teh H S, Kishi H, Scott B, Von Boehmer H
Basel Institute for Immunology, Switzerland.
J Exp Med. 1989 Mar 1;169(3):795-806. doi: 10.1084/jem.169.3.795.
Transgenic mice that carry on a large fraction of their T cells an alpha/beta T cell receptor that recognizes the male antigen in the context of H-2Db molecules were constructed. An mAb specific for the transgenic receptor was developed and used to analyze T cell subsets in male transgenic H-2b mice. The vast majority of immature CD4+8+ T cells that express the transgenic TCR were deleted in the male transgenic mouse. Nevertheless, the majority of T cells spared by this deletion process expressed a high level of the transgenic TCR. These T cells, however, had an abnormal CD4/CD8 phenotype in that they expressed either no CD8 molecules or only low levels.
构建了转基因小鼠，其大部分T细胞携带一种α/βT细胞受体，该受体在H-2Db分子的背景下识别雄性抗原。开发了一种针对转基因受体的单克隆抗体，并用于分析雄性转基因H-2b小鼠中的T细胞亚群。在雄性转基因小鼠中，绝大多数表达转基因TCR的未成熟CD4+8+T细胞被删除。然而，在此删除过程中存活下来的大多数T细胞表达高水平的转基因TCR。然而，这些T细胞具有异常的CD4/CD8表型，即它们要么不表达CD8分子，要么仅表达低水平的CD8分子。
