Zaki Moushira Erfan, El-Bassyouni Hala, Kamal Sanaa, El-Gammal Mona, Youness Eman
Department of Biological Anthropology, National Research Center, Cairo, Egypt.
Department of Clinical Genetics, National Research Center, Cairo, Egypt.
Indian J Endocrinol Metab. 2014 May;18(3):340-4. doi: 10.4103/2230-8210.131173.
The aim of the present study was to investigate the serum paraoxonase 1 (PON1) concentration and oxidative stress markers and assess its relations with the biochemical parameters in obese adolescents.
One hundred and fifty obese adolescents (range 16-18 years) and 150 healthy age- and sex-matched controls were enrolled in the study. The data were extracted from a project entitled "Obesity among Youth: Lifestyle and Genetic Factors" funded by the Science and Technology Development Fund, Egypt. Serum paraoxonase 1 (PON1), nitric oxide (NO), and malonaldehyde were measured. Anthropometry, fasting glucose, insulin concentrations, total cholesterol, high density lipoprotein-cholesterol, low density lipoprotein-cholesterol, triglycerides, systolic and diastolic blood pressure (BP) were measured. Insulin resistance was determined by Homeostasis Model Assessment of Insulin Resistance (HOMA-IR). Diagnostic accuracy of oxidative markers to identify dyslipidemia was calculated with ROC analysis.
The study showed that PON1 activity was significantly lower in obese adolescents than controls. Obese adolescents had significant lower NO level and significant increased MA values as compared to controls. PON1 was negatively correlated with MAD and body mass index in obese subjects. Obese adolescents showed dyslipidemia and increased blood pressure and HOMA-IR values. PON1 had high area under the curve in ROC analysis for identifying dyslipidemia in obese subjects.
Our results indicate that obese subjects have increased oxidative stress and decreased PON1 activity. The lower paraoxonase level might contribute to the greater risk of dyslipidemia, insulin resistance, high blood pressure that are considered as important components in the pathogenesis of the metabolic syndrome in obese adolescents.
本研究旨在调查肥胖青少年血清对氧磷酶1（PON1）浓度和氧化应激标志物，并评估其与生化参数的关系。
本研究纳入了150名肥胖青少年（年龄范围16 - 18岁）和150名年龄及性别匹配的健康对照者。数据取自由埃及科学技术发展基金资助的名为“青少年肥胖：生活方式和遗传因素”的项目。检测血清对氧磷酶1（PON1）、一氧化氮（NO）和丙二醛。测量人体测量学指标、空腹血糖、胰岛素浓度、总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、甘油三酯、收缩压和舒张压（BP）。通过胰岛素抵抗稳态模型评估（HOMA - IR）确定胰岛素抵抗。采用ROC分析计算氧化标志物识别血脂异常的诊断准确性。
研究表明，肥胖青少年的PON1活性显著低于对照组。与对照组相比，肥胖青少年的NO水平显著降低，MA值显著升高。在肥胖受试者中，PON1与MAD和体重指数呈负相关。肥胖青少年表现出血脂异常、血压升高和HOMA - IR值升高。在ROC分析中，PON1在识别肥胖受试者血脂异常方面曲线下面积较大。
我们的结果表明，肥胖受试者氧化应激增加，PON1活性降低。对氧磷酶水平较低可能导致肥胖青少年患血脂异常、胰岛素抵抗和高血压的风险增加，这些被认为是肥胖青少年代谢综合征发病机制中的重要组成部分。
