Universidade Federal de Pelotas (UFPel), Faculdade de Nutrição, Pelotas, RS, Brazil.
Universidade Federal de Pelotas (UFPel), Hospital Universitário, Pelotas, RS, Brazil.
J Pediatr (Rio J). 2020 Jul-Aug;96(4):495-502. doi: 10.1016/j.jped.2019.02.004. Epub 2019 Apr 18.
The cardioprotective enzyme paraoxonase-1 (PON1) suffers an important influence from genetic polymorphisms and nutritional factors. The aim of this study was to investigate the influence of diet, nutritional status, and the C(-107)T polymorphism on PON1 arylesterase activity in children.
This was a cross-sectional study with 97 children, aged between 5 and 8 years, of both genders, from a pediatric outpatient clinic in southern Brazil. A sociodemographic, behavioral, and food consumption questionnaire was applied, and anthropometric measurements and laboratory blood samples were taken. PON1 arylesterase activity was measured by phenol extinction (U/mL), and DNA extraction and analysis of the PON1 C(-107)T polymorphism were performed. The Hardy-Weinberg equilibrium was tested with the chi-squared test and linear regression was used to estimate PON1 activity according to four adjustment models, with an acceptable error of 5%.
In the sample, the male gender accounted for 50.5%, 39.2% were 6 years of age, 54.5% had normal weight, and 51.5% had PON1 activity below the median (90.0, 15-30U/mL). Genotype frequency was 54.6% (53/97), 31.0% (30/97), and 14.4% (14/97), respectively, for CT, CC, and TT, consistent with the Hardy-Weinberg equilibrium (p=0.22). In the regression analysis, the model that included sociodemographic variables as well as frequency of consumption of fruits, vegetables, legumes, dairy products, and beans estimated a variability of 14.8% in PON1 activity combined with the PON1 C(-107)T polymorphism.
During childhood, a good-quality diet with greater inclusion of healthy foods was important to predict the activity of the cardioprotective enzyme PON1 combined with the C(-107)T polymorphism of the PON1 gene.
保护心脏的酶对氧磷酶-1（PON1）受到遗传多态性和营养因素的重要影响。本研究旨在探讨饮食、营养状况和 C（-107）T 多态性对儿童 PON1 芳基酯酶活性的影响。
这是一项横断面研究，共纳入 97 名 5-8 岁的巴西南部儿科门诊的儿童，男女不限。应用社会人口学、行为和食物消费问卷，并进行人体测量学测量和实验室血液样本采集。通过酚消光法（U/mL）测量 PON1 芳基酯酶活性，提取 DNA 并分析 PON1 C（-107）T 多态性。用卡方检验检验 Hardy-Weinberg 平衡，用线性回归根据四个调整模型估计 PON1 活性，误差可接受值为 5%。
在样本中，男性占 50.5%，39.2%为 6 岁，54.5%为正常体重，51.5%的 PON1 活性低于中位数（90.0，15-30U/mL）。基因型频率分别为 54.6%（53/97）、31.0%（30/97）和 14.4%（14/97），分别为 CT、CC 和 TT，与 Hardy-Weinberg 平衡一致（p=0.22）。在回归分析中，包含社会人口学变量以及水果、蔬菜、豆类、乳制品和豆类消费频率的模型，结合 PON1 C（-107）T 多态性，估计 PON1 活性的变异性为 14.8%。
在儿童期，饮食质量较好，更多地摄入健康食品，对预测与 PON1 基因 C（-107）T 多态性相结合的保护性酶 PON1 的活性很重要。
