Albayrak Sevil, Aksoy Ahmet, Yurtseven Lutfiye, Yaşar Abit
Science Faculty, Department of Biology, Erciyes University, Kayseri, Turkey.
J Pharm Pharmacol. 2014 Nov;66(11):1631-40. doi: 10.1111/jphp.12288. Epub 2014 Jun 19.
The phenolic components and biological activity of nine Senecio species growing in Turkey were investigated.
Senecio species were extracted with methanol. The content of total phenols was determined using Folin-Ciocalteu method, while individual phenolic acids and flavonoids were detected using HPLC analysis. Also, to determine the antioxidant capacity, phosphomolybdenum assay and 1,1-diphenyl picrylhydrazyl (DPPH) radical-scavenging activity assay were used. Antimicrobial activity of extract was determined using agar diffusion and broth microdilution method.
The total phenolic contents of the extracts were found to be highest in Senecio cilicius and Senecio mollis extracts (117.45 and 113.40 mg equivalent to gallic acid/g, respectively). S. salsuginea showed the strongest free radical-scavenging activity with IC50 (the concentration providing 50% inhibition) = 26.23 μg/ml and S. mollis showed the highest antioxidant capacity in the phosphomolybdenum method (434.48 mg equivalent to ascorbic acid/g). The extracts exerted promising antibacterial activity against most of the test bacteria (minimal inhibitory concentration = 6.25-12.5 mg/ml), but no activity was observed against Candida albicans.
The results demonstrated that nine Senecio species possess high antioxidant and antimicrobial activity in accordance with the high amount of phenolic contents in the extracts that might be natural agencies used in many areas such as food, pharmacy and alternative medicine.
对生长在土耳其的九种千里光属植物的酚类成分及生物活性进行研究。
用甲醇对千里光属植物进行提取。采用福林-酚法测定总酚含量，同时用高效液相色谱分析法检测各酚酸和黄酮类化合物。此外，为测定抗氧化能力，采用了磷钼酸法和1,1-二苯基-2-苦基肼（DPPH）自由基清除活性测定法。提取物的抗菌活性采用琼脂扩散法和肉汤微量稀释法测定。
提取物中总酚含量在西里西亚千里光和软毛千里光提取物中最高（分别为117.45和113.40毫克没食子酸当量/克）。盐生千里光的自由基清除活性最强，IC50（产生50%抑制作用的浓度）=26.23微克/毫升，软毛千里光在磷钼酸法中抗氧化能力最高（434.48毫克抗坏血酸当量/克）。提取物对大多数受试细菌具有良好的抗菌活性（最低抑菌浓度=6.25-12.5毫克/毫升），但对白色念珠菌无活性。
结果表明，九种千里光属植物具有较高的抗氧化和抗菌活性，这与提取物中高含量的酚类成分一致，这些植物可能是食品、制药和替代医学等许多领域中使用的天然原料。
