Alqahtani Ali S, Herqash Rashed N, Noman Omar M, Nasr Fahd A, Alyhya Nouf, Anazi Shamsa H, Farooq Muhammad, Ullah Riaz
Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Medicinal, Aromatic and Poisonous Plants Research Center, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
Evid Based Complement Alternat Med. 2020 Oct 24;2020:8875430. doi: 10.1155/2020/8875430. eCollection 2020.
Current treatments for complex diseases have remarkable side effects that negatively impact patients' quality of life. Thus, natural compounds with fewer side effects represent a promising source for safe drugs. The genus is widely used in folk medicine due to its various pharmacological properties. In the present study, the total phenolic content of , which is grown in Saudi Arabia, was assessed using the Folin-Ciocalteau colorimetric method. Scavenging DPPH and ABTS assays were utilized to determine the antioxidant properties of fractions, and MTT assay was used to screen the cytotoxic activity of against various cancer cells. In addition, HPLC-UV was utilized to detect the presence of two phenolic acids, namely, vanillic acid (VA) and gallic acid (GA). Among all fractions tested, chloroform fraction (SGCF) yielded the highest value (125.3 mg·GA/g) in terms of total phenolic content. SGCF also exhibited the highest scavenging activities (76.7 and 74.1%) on both DPPH and ABTS assays, respectively. Similarly, SGCF also possessed the most potent cytotoxic activity against the MCF-7 cell line, with an IC value of 41.8 g/ml. The validated HPLC method confirmed the presence of VA (4.8 g/mg DW) and GA (3.9 g/mg DW) in SGCF. Overall, our data show that had antioxidant and cytotoxic properties. A developed validated HPLC method which could be helpful for quantifying phenolic compounds in was established.
目前针对复杂疾病的治疗方法具有显著的副作用，会对患者的生活质量产生负面影响。因此，副作用较少的天然化合物是安全药物的一个有前景的来源。该属植物因其多种药理特性而在民间医学中广泛使用。在本研究中，采用福林-西奥尔特法比色法评估了生长在沙特阿拉伯的该植物的总酚含量。利用清除DPPH和ABTS实验来测定该植物提取物的抗氧化性能，并用MTT实验筛选该植物对各种癌细胞的细胞毒性活性。此外，利用高效液相色谱-紫外检测法检测两种酚酸，即香草酸（VA）和没食子酸（GA）的存在。在所有测试的提取物中，氯仿提取物（SGCF）的总酚含量最高（125.3 mg·GA/g）。SGCF在DPPH和ABTS实验中的清除活性也分别最高（76.7%和74.1%）。同样，SGCF对MCF-7细胞系也具有最强的细胞毒性活性，IC值为41.8 μg/ml。经过验证的高效液相色谱法证实了SGCF中存在VA（4.8 μg/mg DW）和GA（3.9 μg/mg DW）。总体而言，我们的数据表明该植物具有抗氧化和细胞毒性特性。建立了一种经过验证的高效液相色谱法，该方法有助于定量该植物中的酚类化合物。
