[参芎注射液对大鼠脑缺血再灌注损伤的保护作用]

[Protective effect of Shenxiong injection on cerebral ischemia-reperfusion injury of rats].

作者信息

Wang Xing-Yu, Zhang Yu-Yan, Wan Hai-Tong, Zhao Tao, Fu Wei, Zhou Hui-Fen, Zhou Peng, Wang Ze-Feng, Yang Jie-Hong

出版信息

Zhongguo Zhong Yao Za Zhi. 2014 Feb;39(3):503-6.

PMID:24946556

Abstract

OBJECTIVE

To study the protective effect of Shenxiong injection on the cerebral ischemia-reperfusion injury of senile rats.

METHOD

Totally 108 Sprague-Dawley (SD) rats were randomly divided into the sham operation group, the model group, the Ni-modipine group and Shenxiong injection groups (low, middle, and high doses). The rat brain ischemia-reperfusion model was established by the middle cerebral artery occlusion (MCAO) method in rats, in order to observe the effect of Shenxiong injection on neurological score and brain infarct volume of rats with cerebral ischemia-reperfusion injury, and determine the contents of NOS, NO, SOD, MDA and LDH in brain tissues. The contents of TNF-alpha and IL-1beta levels in brain tissues were measured by enzyme-linked immunosorbent assay (ELISA) method.

RESULT

Shenxiong injection could significantly decrease neurological score, injury degree of brain tissues and brain infarct volume of rats with cerebral ischemia-reperfusion injury, increase the vigor of SOD, decrease the levels of MDA, NO, NOS and LDH, and inhibit IL-1beta and TNF-alpha expressions.

CONCLUSION

Shenxiong injection has the obvious protective effect on the brain ischemia-reperfusion injury in rats. Its mechanism may be related to the improvement of neurological function, the reduction of free radical injury, and the inhibition of inflammation factor expression.

摘要

目的

研究参芎注射液对老年大鼠脑缺血再灌注损伤的保护作用。

方法

将108只Sprague-Dawley（SD）大鼠随机分为假手术组、模型组、尼莫地平组和参芎注射液组（低、中、高剂量）。采用大鼠大脑中动脉闭塞（MCAO）法建立大鼠脑缺血再灌注模型，观察参芎注射液对脑缺血再灌注损伤大鼠神经功能评分及脑梗死体积的影响，并测定脑组织中一氧化氮合酶（NOS）、一氧化氮（NO）、超氧化物歧化酶（SOD）、丙二醛（MDA）和乳酸脱氢酶（LDH）的含量。采用酶联免疫吸附测定（ELISA）法检测脑组织中肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）水平。