Pang Xiao-Bin, Xie Xin-Mei, Wang Hai-Yan, Wang Bao-Quan
Zhongguo Zhong Yao Za Zhi. 2014 Feb;39(4):721-5.
To discuss the protective effect of Mailuoning injection on ischemia/reperfusion (I/R) injury in rats and its mechanism.
Healthy male adult Sprague-Dawley (SD) rats were randomly divided into the sham operation group, the model group, the edaravone (3 mg x kg(-1)) control group, and Mailuoning high, middle and low-dose groups (4, 2, 1 mL x kg(-1)), with 10 rats in each group, and administered with drugs through tail intravenous injection. The middle cerebral artery occlusion (MCAO) was adopted to establish the rat ischemia/reperfusion model. After the ischemia for 2 h and reperfusion for 24 h, the pathological changes in neurovascular units (NVU) of brain tissues at the ischemia side was observed by HE staining. The expressions of glialfibrillary acidic protein (GFAP) and ionized calcium-binding adaptor molecule 1 (Ibal) were detected by the immunohistochemical method. The expressions of tumor necrosis factor-alpha (TNF-alpha), interleukin 1beta (IL-1beta), vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) were detected by the western blotting technique.
Mailuoning injection could significantly improve the pathological changes in cortical penumbra brain tissue UVN of (I/R) rats, reduce the number of GFAP and Ibal positive cells, and significantly decrease the expressions of TNF-alpha, IL-1beta, VCAM-1 and ICAM-1 of brain tissues of I/R rats.
Mailuoning injection shows an obvious protective effect on UVN of I/R rats. Its mechanism may involve the inhibition of the activation of astrocyte and microglia and the secretion and expression of various inflammatory factors.
探讨脉络宁注射液对大鼠缺血/再灌注（I/R）损伤的保护作用及其机制。
将健康雄性成年Sprague-Dawley（SD）大鼠随机分为假手术组、模型组、依达拉奉（3 mg·kg⁻¹）对照组、脉络宁高、中、低剂量组（4、2、1 mL·kg⁻¹），每组10只，通过尾静脉注射给药。采用大脑中动脉闭塞（MCAO）法建立大鼠缺血/再灌注模型。缺血2 h再灌注24 h后，通过HE染色观察缺血侧脑组织神经血管单元（NVU）的病理变化。采用免疫组织化学方法检测胶质纤维酸性蛋白（GFAP）和离子钙结合衔接分子1（Iba1）的表达。采用蛋白质印迹技术检测肿瘤坏死因子-α（TNF-α）、白细胞介素1β（IL-1β）、血管细胞黏附分子-1（VCAM-1）和细胞间黏附分子-1（ICAM-1）的表达。
脉络宁注射液可显著改善I/R大鼠皮质半暗带脑组织UVN的病理变化，减少GFAP和Iba1阳性细胞数量，并显著降低I/R大鼠脑组织TNF-α、IL-1β、VCAM-1和ICAM-1的表达。
脉络宁注射液对I/R大鼠的UVN具有明显的保护作用。其机制可能涉及抑制星形胶质细胞和小胶质细胞的活化以及多种炎症因子的分泌和表达。
