Peng An-Ping, Wang Xiao-Yun, Zhuang Jun-Hua
Zhongguo Zhong Yao Za Zhi. 2014 Feb;39(3):536-9.
Triptolide (TPT), an active compound extracted from Chinese herb Tripterygium wilfordii , has been used in therapy of rheumatoid arthritis (RA). In this study, after synovial fibroblasts from rheumatoid arthritis (RASFs) were treated with TPT, we investigated its effect on the differentiation of Th17 cells. Firstly, the mRNA level of cyclooxygenase (COX) wad detected by qRT-PCR and the protein level of prostaglandin E2 (PGE2) was tested by ELISA in RASFs treated with different concentrations (0, 10, 50, 100 nmol L-1 ) of TPT. Then after TPT pre-treated RASFs and RA CD4 + T cells wer e co-cultured for 3 days in the presence or absence of PGE2, IL-17 and IFN-gamma production in CD4 T cell subsets were detected by flow cytometry. The results showed TPT decreased the mRNA experssion of COX2 and the secretion of PGE2 in RASFs in a dose-dependent manner(P <0. 05). We further found that differentiation of Thl7 cells was downregulated in a dose-dependent manner, and exogenous PGE2 could reverse the inhibition of Th17 cell differentiation(P <0. 05). Taken together, our results demonstrated that TPT inhibited the mRNA level of COX2 and the secretion of PGE2 in RASFs, which partly led to impaired Th17 cell differentiation in vitro.
雷公藤甲素（TPT）是从中药雷公藤中提取的一种活性化合物，已用于类风湿性关节炎（RA）的治疗。在本研究中，用TPT处理类风湿性关节炎滑膜成纤维细胞（RASFs）后，我们研究了其对Th17细胞分化的影响。首先，通过qRT-PCR检测不同浓度（0、10、50、100 nmol L-1）TPT处理的RASFs中环氧合酶（COX）的mRNA水平，并用ELISA检测前列腺素E2（PGE2）的蛋白水平。然后，在有或无PGE2的情况下，将TPT预处理的RASFs与RA CD4 + T细胞共培养3天，通过流式细胞术检测CD4 T细胞亚群中IL-17和IFN-γ的产生。结果表明，TPT以剂量依赖性方式降低RASFs中COX2的mRNA表达和PGE2的分泌（P <0.05）。我们进一步发现，Thl7细胞的分化以剂量依赖性方式下调，外源性PGE2可逆转对Th17细胞分化的抑制作用（P <0.05）。综上所述，我们的结果表明，TPT抑制RASFs中COX2的mRNA水平和PGE2的分泌，这在一定程度上导致体外Th17细胞分化受损。
