Chen Li-Hong, Liu Zheng-Tao, Fang Zheng, Wang Xiao-Nan, Wang Wan-Hua
Huan Jing Ke Xue. 2014 Apr;35(4):1486-90.
In this study, effects of Pb (NO3)2 on the growth and reproduction of Eisenia fetida were investigated with artificial soils. The results showed that: compared with the control group, the growth of E. fetida in contaminated soil was inhibited significantly with increasing Pb (NO3)2 concentrations. But there were great differences in the extent of inhibition after 0, 7, 28 and 84 days of aging in the artificial soils. The most obvious effect occurred in 1000 mg x kg(-1) and 1500 mg x kg(-1) experimental groups. There was a very big difference in the effects of Pb (NO3)2 on reproduction of E. fetida with different aging times. The medium effective concentration (EC50)values of cocoon production inhibition were 1085.10, 1163.32, 1666.86 and 1606.94 mg x kg(-1). In addition, the EC50 values of the number of juvenile inhibition were 700.01, 703.88, 1 031.34 and 1 017.89 mg x kg(-1), respectively. This study indicates that soil aging which affects the toxicity of Pb (NO3)2 should be taken into consideration when toxicity tests of Pb (NO3)2 were carried out. The results could provide a scientific basis for the ecological risk assessment of aging soils and soil environmental quality standards.
本研究采用人工土壤，研究了Pb(NO₃)₂对赤子爱胜蚓生长和繁殖的影响。结果表明：与对照组相比，随着Pb(NO₃)₂浓度增加，污染土壤中赤子爱胜蚓的生长受到显著抑制。但在人工土壤中老化0、7、28和84天后，抑制程度存在很大差异。最明显的影响出现在1000mg·kg⁻¹和1500mg·kg⁻¹实验组。不同老化时间下，Pb(NO₃)₂对赤子爱胜蚓繁殖的影响差异很大。茧产量抑制的半数效应浓度(EC₅₀)值分别为1085.10、1163.32、1666.86和1606.94mg·kg⁻¹。此外，幼蚓数量抑制的EC₅₀值分别为700.01、703.88、1031.34和1017.89mg·kg⁻¹。本研究表明，在进行Pb(NO₃)₂毒性试验时，应考虑影响Pb(NO₃)₂毒性的土壤老化因素。研究结果可为老化土壤的生态风险评估和土壤环境质量标准提供科学依据。
