Milberg Oleg, Tora Muhibullah, Shitara Akiko, Takuma Taishin, Masedunskas Andrius, Weigert Roberto
Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Dr. 303A, 20892-4340, Bethesda, MD, USA.
Methods Mol Biol. 2014;1174:407-21. doi: 10.1007/978-1-4939-0944-5_28.
The actin cytoskeleton plays a fundamental role in controlling several steps during regulated exocytosis. Here, we describe a combination of procedures that are aimed at studying the dynamics and the mechanism of the actin cytoskeleton in the salivary glands of live rodents, a model for exocrine secretion. Our approach relies on intravital microscopy, an imaging technique that enables imaging biological events in live animals at a subcellular resolution, and it is complemented by the use of pharmacological agents and indirect immunofluorescence in the salivary tissue.
肌动蛋白细胞骨架在调节性胞吐作用的多个步骤控制中发挥着基本作用。在此，我们描述了一系列方法的组合，旨在研究活啮齿动物唾液腺中肌动蛋白细胞骨架的动力学和机制，唾液腺是外分泌分泌的一个模型。我们的方法依赖于活体显微镜检查，这是一种能够以亚细胞分辨率对活体动物中的生物事件进行成像的技术，并辅以在唾液组织中使用药理试剂和间接免疫荧光。
