Stalmach Angélique, Williamson Gary, Crozier Alan
Plant Products and Human Nutrition Group, Joseph Black Building, School of Medicine, College of Biomedical, Veterinary and Life Sciences, University of Glasgow, UK.
Food Funct. 2014 Aug;5(8):1727-37. doi: 10.1039/c4fo00316k.
Single servings of coffee beverage containing low (412 μmol), medium (635 μmol) and high (795 μmol) amounts of chlorogenic acids were administered to eleven healthy volunteers in a double-blind randomised controlled trial. Analysis of plasma and urine collected for 24 h revealed the presence of 12 metabolites in plasma and 16 metabolites in urine, principally in the form of sulphates, and to a lesser extent glucuronides of caffeic, ferulic, dihydrocaffeic and dihydroferulic acids, as well as intact feruloylquinic and caffeoylquinic acids, and sulphated caffeoylquinic acid lactones. Median values of peak plasma concentrations after increasing doses of chlorogenic acids were 1088, 1526 and 1352 nM. In urine the median amounts of metabolites excreted after 24 h following consumption of the three coffees were 101, 160 and 125 μmol, accounting for 24%, 25% and 16% of the doses ingested. Peak plasma concentration and urinary excretion values showed trends towards a reduced bioavailability of chlorogenic acids associated with the highest dose ingested, when expressed as percentages of intake. Potential biomarkers of coffee intake were identified as feruloylquinic acids and sulphated caffeoylquinic acid lactones in plasma and urine with positive moderate to strong coefficients of determination for peak plasma concentrations (0.60-0.81) and amounts excreted in urine (0.36-0.73) (P < 0.05).
在一项双盲随机对照试验中，向11名健康志愿者提供了单份分别含有低量（412微摩尔）、中量（635微摩尔）和高量（795微摩尔）绿原酸的咖啡饮品。对收集24小时的血浆和尿液进行分析发现，血浆中存在12种代谢物，尿液中存在16种代谢物，主要以硫酸盐形式存在，其次是咖啡酸、阿魏酸、二氢咖啡酸和二氢阿魏酸的葡萄糖醛酸苷，以及完整的阿魏酰奎尼酸和咖啡酰奎尼酸，还有硫酸化的咖啡酰奎尼酸内酯。随着绿原酸剂量增加，血浆峰值浓度的中位数分别为1088、1526和1352纳摩尔。饮用三种咖啡后24小时，尿液中代谢物排泄量的中位数分别为101、160和125微摩尔，分别占摄入剂量的24%、25%和16%。以摄入量的百分比表示时，血浆峰值浓度和尿液排泄值显示出与最高摄入剂量相关的绿原酸生物利用度降低的趋势。咖啡摄入量的潜在生物标志物被确定为血浆和尿液中的阿魏酰奎尼酸和硫酸化咖啡酰奎尼酸内酯，其对血浆峰值浓度（0.60 - 0.81）和尿液排泄量（0.36 - 0.73）具有正的中度至强决定系数（P < 0.05）。
