Heymann Paul G B, Mandic Robert, Kämmerer Peer W, Kretschmer Frank, Saydali Akram, Neff Andreas, Draenert Florian G
Department of Oral and Maxillofacial Surgery, University of Marburg, University Hospital Giessen and Marburg, Campus Marburg, Baldingerstraße, D-35037 Marburg, Germany.
Department of Otorhinolaryngology, University of Marburg, University Hospital Giessen and Marburg, Campus Marburg, Baldingerstraße, D-35037 Marburg, Germany.
J Craniomaxillofac Surg. 2014 Oct;42(7):1469-74. doi: 10.1016/j.jcms.2014.04.014. Epub 2014 May 10.
Low-level laser therapy (LLLT) is used in parodontitis treatment in combination with an antimicrobial photosensitizer. The purpose of this study was to investigate the combination of LLLT with cisplatin and zoledronic acid as potential photosensitizer in-vitro.
Primary human fibroblasts (PHF) and head and neck squamous cell carcinoma cells (HNSCC, exactly UM-SCC-3) were treated with different concentrations of zoledronatic acid and cisplatin and irradiated twice with a diode laser (wavelength 670 nm, 2 min). Cell viability was tested by XTT assay and histomorphological analysis with HE staining.
LLLT increased bioviability for both cell lines (p < 0.001). LLLT lowered PHF viability at the highest concentrations of cisplatin (p = 0.027 and p = 0.005) and zoledronic acid (p < 0.001). For HNSCCs, LLLT reduced cell viability at every concentration of cisplatin (all p < 0.05). In cases of incubation with zoledronic acid, similar to fibroblasts, laser therapy lowered cell viability at the highest concentration only (p < 0.001).
Within the limits of this study, it can be concluded that LLLT enhances the effect of cisplatin and zoledronic acid in the discussed cells in order to develop new therapeutic options for cysts in the cranio-maxillofacial region and other appropriate indications.
低强度激光疗法（LLLT）与抗菌光敏剂联合用于牙周炎治疗。本研究的目的是在体外研究LLLT与顺铂和唑来膦酸作为潜在光敏剂的联合应用。
用不同浓度的唑来膦酸和顺铂处理原代人成纤维细胞（PHF）和头颈部鳞状细胞癌细胞（HNSCC，确切地说是UM-SCC-3），并用二极管激光（波长670nm，照射2分钟）照射两次。通过XTT法和苏木精-伊红（HE）染色的组织形态学分析来检测细胞活力。
LLLT提高了两种细胞系的生物活性（p < 0.001）。在顺铂（p = 0.027和p = 0.005）和唑来膦酸（p < 0.001）的最高浓度下，LLLT降低了PHF的活力。对于HNSCC，在顺铂的每个浓度下，LLLT都降低了细胞活力（所有p < 0.05）。在用唑来膦酸孵育的情况下，与成纤维细胞类似，激光疗法仅在最高浓度下降低了细胞活力（p < 0.001）。
在本研究的范围内，可以得出结论，LLLT增强了顺铂和唑来膦酸在所讨论细胞中的作用，以便为颅颌面区域的囊肿和其他合适适应症开发新的治疗选择。
