Franc Martin, Sobotníková Jana, Coufal Pavel, Bosáková Zuzana
Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov, Prague, Czech Republic.
J Sep Sci. 2014 Sep;37(17):2278-83. doi: 10.1002/jssc.201400434. Epub 2014 Jul 21.
Fused-silica capillary columns for high-performance liquid chromatography with 320 and 250 μm inner diameter were prepared by slurry packing with 5 and 3 μm Nucleosil C18 stationary phase. Different types of mechanical and monolithic outlet frits were used and their influence on the resulting column performance was evaluated. Columns with quartz wool exhibited symmetrical peaks and low theoretical plate height, and the preparation time was short. The performance of monolithic frits varied based on type of monolith, length of the frit, and silanization procedure. The best frit performed similarly to the quartz wool ones, but the preparation took several hours. Their main advantage lies in the possibility of on-column detection, because the detection window can be burnt immediately behind the frit.
采用5μm和3μm的Nucleosil C18固定相通过匀浆法填充制备了内径为320μm和250μm的高效液相色谱熔融石英毛细管柱。使用了不同类型的机械型和整体式出口筛板，并评估了它们对所得柱性能的影响。装有石英棉的色谱柱呈现对称峰且理论塔板高度较低，并且制备时间较短。整体式筛板的性能因整体类型、筛板长度和硅烷化程序而异。性能最佳的筛板与装有石英棉的筛板表现相似，但制备需要几个小时。它们的主要优点在于可以进行柱上检测，因为检测窗口可以直接在筛板后方烧制。
