Baba Koji, Arao Tomohito, Yamaguchi Noriko, Watanabe Eiki, Eun Heesoo, Ishizaka Masumi
National Institute for Agro-Environmental Sciences, Tsukuba 305-8604, Ibaraki, Japan.
National Institute for Agro-Environmental Sciences, Tsukuba 305-8604, Ibaraki, Japan.
J Chromatogr A. 2014 Aug 8;1354:109-16. doi: 10.1016/j.chroma.2014.05.072. Epub 2014 Jun 4.
Arsenic species, including arsenous acid, arsenic acid, methylarsonic acid, and dimethylarsinic acid, were determined using HPLC-ICPMS. The species were separated with a Discovery HS F5 column and a simple, volatile, and isocratic mobile phase of 0.1% (v/v) formic acid and 1% (v/v) methanol. The Discovery HS F5 column with a pentafluorophenyl (PFP) stationary phase gave sharp peaks and full separation of the arsenic species in 5min, and other PFP columns showed lower performance. This separation method was applied to arsenic species analysis in rice. The extraction of arsenic from rice samples was performed using 0.15M nitric acid. The methodology was validated by use of certified reference materials, NMIJ CRM 7503-a and NIST SRM 1568a, and extremely low arsenic rice samples as blank samples.
